Anti-ANKK1 antibody (ab219189)

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Overview

  • Product name

    Anti-ANKK1 antibody
    See all ANKK1 primary antibodies

  • Description

    Goat polyclonal to ANKK1

  • Host species

    Goat

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Monkey, Gorilla

  • Immunogen

    Synthetic peptide corresponding to Human ANKK1 aa 482-496 (internal sequence) (Cysteine residue). (NP_848605.1)
    Sequence:

    SRQADPNLHEAEGKT


    Database link: Q8NFD2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human prostate tissue; Human Brain (Substantia nigra and Amygdala) lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab219189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35, 85 kDa (predicted molecular weight: 85 kDa).

Target

  • Relevance

    ANKK1 belongs to the TKL Ser/Thr protein kinase superfamily, contains twelve ANK repeats and one protein kinase domain. The protein is weakly expressed in testis, placenta, and spinal cord.

  • Database links

    • Alternative names

      • Ankyrin repeat and kinase domain containing 1 antibody
      • Ankyrin repeat and protein kinase domain containing protein 1 antibody
      • EC 2.7.11.1 antibody
      • PKK2 antibody
      • Protein kinase PKK2 antibody
      • SGK288 antibody
      • Sugen kinase 288 antibody
      • X kinase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKK1 antibody (ab219189)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKK1 antibody (ab219189)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling ANKK1 with ab219189 at 10 µg/ml.

    • Western blot - Anti-ANKK1 antibody (ab219189)
      Western blot - Anti-ANKK1 antibody (ab219189)
      Anti-ANKK1 antibody (ab219189) at 0.1 µg/ml + Human Brain (Substantia nigra and Amygdala) lysates in RIPA buffer at 35 µg/ml

      Developed using the ECL technique.

      Predicted band size: 85 kDa



      An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide.

    References

    ab219189 has not yet been referenced specifically in any publications.

    Publishing research using ab219189? Please let us know so that we can cite the reference in this datasheet.

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