Anti-ANKK1 antibody (ab219189)
Key features and details
- Goat polyclonal to ANKK1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKK1 antibody
See all ANKK1 primary antibodies -
Description
Goat polyclonal to ANKK1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human ANKK1 aa 482-496 (internal sequence) (Cysteine residue). (NP_848605.1)
Sequence:SRQADPNLHEAEGKT
Database link: Q8NFD2 -
Positive control
- Human prostate tissue; Human Brain (Substantia nigra and Amygdala) lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab219189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35, 85 kDa (predicted molecular weight: 85 kDa). |
Target
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Relevance
ANKK1 belongs to the TKL Ser/Thr protein kinase superfamily, contains twelve ANK repeats and one protein kinase domain. The protein is weakly expressed in testis, placenta, and spinal cord. -
Database links
- Entrez Gene: 785303 Cow
- Entrez Gene: 489403 Dog
- Entrez Gene: 100054819 Horse
- Entrez Gene: 255239 Human
- Omim: 608774 Human
- SwissProt: Q8NFD2 Human
- Unigene: 448473 Human
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Alternative names
- Ankyrin repeat and kinase domain containing 1 antibody
- Ankyrin repeat and protein kinase domain containing protein 1 antibody
- EC 2.7.11.1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKK1 antibody (ab219189)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling ANKK1 with ab219189 at 10 µg/ml.
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Western blot - Anti-ANKK1 antibody (ab219189)Anti-ANKK1 antibody (ab219189) at 0.1 µg/ml + Human Brain (Substantia nigra and Amygdala) lysates in RIPA buffer at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 85 kDaAn additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Protocols
Datasheets and documents
References (0)
ab219189 has not yet been referenced specifically in any publications.
