Key features and details
- Goat polyclonal to ANKK1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKK1 antibody
See all ANKK1 primary antibodies
DescriptionGoat polyclonal to ANKK1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Monkey, Gorilla
- Human prostate tissue; Human Brain (Substantia nigra and Amygdala) lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35, 85 kDa (predicted molecular weight: 85 kDa).|
RelevanceANKK1 belongs to the TKL Ser/Thr protein kinase superfamily, contains twelve ANK repeats and one protein kinase domain. The protein is weakly expressed in testis, placenta, and spinal cord.
- Ankyrin repeat and kinase domain containing 1 antibody
- Ankyrin repeat and protein kinase domain containing protein 1 antibody
- EC 126.96.36.199 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling ANKK1 with ab219189 at 10 µg/ml.
Anti-ANKK1 antibody (ab219189) at 0.1 µg/ml + Human Brain (Substantia nigra and Amygdala) lysates in RIPA buffer at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 85 kDa
An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab219189 has not yet been referenced specifically in any publications.