Overview

  • Product name

  • Description

    Rabbit polyclonal to ANKLE2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human ANKLE2 aa 800-850. The exact sequence is proprietary. (NP_055929.1).
    Database link: Q86XL3

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225905 was affinity purified using an epitope specific to ANKLE2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 104 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex.
  • Sequence similarities

    Belongs to the ANKLE2 family.
    Contains 1 ANK repeat.
    Contains 1 LEM domain.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANKL2_HUMAN antibody
    • ANKLE 2 antibody
    • ANKLE2 antibody
    • ankyrin repeat and LEM domain containing 2 antibody
    • Ankyrin repeat and LEM domain-containing protein 2 antibody
    • FLJ 22280 antibody
    • FLJ 36132 antibody
    • FLJ22280 antibody
    • FLJ36132 antibody
    • KIAA 0692 antibody
    • KIAA0692 antibody
    • LEM domain containing 7 antibody
    • LEM domain-containing protein 4 antibody
    • LEMD 7 antibody
    • LEMD7 antibody
    see all

Images

  • All lanes : Anti-ANKLE2 antibody (ab225905) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 104 kDa


    Exposure time: 30 seconds
  • ANKLE2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225905 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225905 at 1 µg/ml.

    Lane 1: ab225905 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab225905 has not yet been referenced specifically in any publications.

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