Key features and details
- Rabbit polyclonal to ANKLE2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKLE2 antibody
See all ANKLE2 primary antibodies
DescriptionRabbit polyclonal to ANKLE2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
Synthetic peptide within Human ANKLE2 aa 800-850. The exact sequence is proprietary. (NP_055929.1).
Database link: Q86XL3
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225905 was affinity purified using an epitope specific to ANKLE2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 104 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionInvolved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex.
Sequence similaritiesBelongs to the ANKLE2 family.
Contains 1 ANK repeat.
Contains 1 LEM domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- ANKL2_HUMAN antibody
- ANKLE 2 antibody
- ANKLE2 antibody
All lanes : Anti-ANKLE2 antibody (ab225905) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 104 kDa
Exposure time: 30 seconds
ANKLE2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225905 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225905 at 1 µg/ml.
Lane 1: ab225905 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225905 has not yet been referenced specifically in any publications.