Key features and details
- Rabbit polyclonal to ANKLE2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKLE2 antibody
See all ANKLE2 primary antibodies
DescriptionRabbit polyclonal to ANKLE2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ANKLE2 (internal sequence).
Database link: Q86XL3
- WB: HEK-293T whole cell lysate. IHC-P: Human breast cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 104 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionInvolved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex.
Sequence similaritiesBelongs to the ANKLE2 family.
Contains 1 ANK repeat.
Contains 1 LEM domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- ANKL2_HUMAN antibody
- ANKLE 2 antibody
- ANKLE2 antibody
Anti-ANKLE2 antibody (ab227538) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 104 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human breast cancer tissue stained for ANKLE2 with ab227538 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227538 has not yet been referenced specifically in any publications.