Key features and details
- Rabbit polyclonal to ANKRD1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKRD1 antibody
See all ANKRD1 primary antibodies
DescriptionRabbit polyclonal to ANKRD1
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human heart muscle tissue. ICC/IF: U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab247009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMay play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.
Tissue specificityMainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells.
Involvement in diseaseDefects in ANKRD1 may be a cause of total anomalous pulmonary venous return (TAPVR) [MIM:106700]. TAPVR is a rare congenital heart disease (CHD) in which the pulmonary veins fail to connect to the left atrium during cardiac development, draining instead into either the right atrium or one of its venous tributaries. This disease accounts for 1.5% of all CHDs and has a prevalence of approximately 1 out of 15'000 live births.
Sequence similaritiesContains 5 ANK repeats.
- Information by UniProt
- ALRP antibody
- ANKR1_HUMAN antibody
- ANKRD 1 antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ANKRD1 using ab247009 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human heart tissue stained for ANKRD1 using ab247009 at 1/20 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for ANKRD1 using ab247009 at 1/20 dilution in immunohistochemical analysis. No positivity in cells in seminiferous ducts as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247009 has not yet been referenced specifically in any publications.