Key features and details
- Rabbit polyclonal to ANKRD10
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKRD10 antibody
DescriptionRabbit polyclonal to ANKRD10
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: RT4 and U-251 MG whole cell lysate; IHC-P: Human stomach tissue; ICC: U2 OS whole cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 45 kDa.|
Sequence similaritiesContains 4 ANK repeats.
- Information by UniProt
- Ankrd10 antibody
- Ankyrin repeat domain-containing protein 10 antibody
- ANR10_HUMAN antibody
- RP11-120J20.3 antibody
Immunocytochemical analysis of permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling ANKRD10 in the cytoplasm and microtubules (green) with ab204396 at 2 µg/ml.
Immunohistochemical analysis of human stomach tissue labeling ANKRD10 in the shows cytoplasm of glandular cells with ab204396 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-ANKRD10 antibody (ab204396) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 45 kDa
ab204396 has not yet been referenced specifically in any publications.