Overview

  • Product name

    Anti-ANKRD11 antibody [2022C8a]
  • Description

    Mouse monoclonal [2022C8a] to ANKRD11
  • Host species

    Mouse
  • Specificity

    This antibody reacts with ANKRD11
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, African green monkey
  • Immunogen

    ANKRD11 recombinant fragment (Human), containing 50-200 aa

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium Azide
    Constituents: 1% PBS, BSA, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Protein G purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    2022C8a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 296 kDa.

Target

  • Relevance

    Ankyrin is a membrane protein that mediates the attachment of the erythrocyte membrane skeleton to the plasma membrane and interacts with CD44 and inositol triphosphate. It contains three functional domains: a conserved N-terminal ankyrin repeat domain (ARD(consisting of 22–24 tandem repeats of 33 amino acids), a spectrin binding domain and a variably sized C-terminal regulatory domain. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops. It has been studied using multiple sequence alignment to determine which conserved amino acid residues are critical for folding and stability. Ankyrin-repeat proteins have been associated with a number of human diseases; most notably, the cell cycle inhibitor p16 is associated with cancer and the Notch protein is a key component of cell signaling pathways whose intracellular repeat domain is disrupted in mutations that give rise to the neurological disorder known as CADASIL.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • ANCO 1 antibody
    • ANCO1 antibody
    • Ankyrin repeat containing cofactor 1 antibody
    • Ankyrin repeat domain 11 antibody
    • Ankyrin repeat domain containing protein 11 antibody
    • LZ16 antibody
    • T13 antibody
    see all

Images

  • Anti-ANKRD11 antibody [2022C8a] (ab50852) at 1/50 dilution + A2058 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody

    Predicted band size: 296 kDa
    Observed band size: >250 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Garee JP  et al. Regulation of HER2 oncogene transcription by a multifunctional coactivator/corepressor complex. Mol Endocrinol 28:846-59 (2014). Read more (PubMed: 24678732) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 13 2007

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 13 2007

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