Key features and details
- Mouse monoclonal [2022C8a] to ANKRD11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ANKRD11 antibody [2022C8a]
DescriptionMouse monoclonal [2022C8a] to ANKRD11
This antibody reacts with ANKRD11.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: BSA, 1% PBS
Concentration information loading...
PurityProtein G purified
Purification notesProtein G purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab50852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Predicted molecular weight: 296 kDa.|
RelevanceAnkyrin is a membrane protein that mediates the attachment of the erythrocyte membrane skeleton to the plasma membrane and interacts with CD44 and inositol triphosphate. It contains three functional domains: a conserved N-terminal ankyrin repeat domain (ARD(consisting of 22–24 tandem repeats of 33 amino acids), a spectrin binding domain and a variably sized C-terminal regulatory domain. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops. It has been studied using multiple sequence alignment to determine which conserved amino acid residues are critical for folding and stability. Ankyrin-repeat proteins have been associated with a number of human diseases; most notably, the cell cycle inhibitor p16 is associated with cancer and the Notch protein is a key component of cell signaling pathways whose intracellular repeat domain is disrupted in mutations that give rise to the neurological disorder known as CADASIL.
- ANCO 1 antibody
- ANCO1 antibody
- Ankyrin repeat containing cofactor 1 antibody
Anti-ANKRD11 antibody [2022C8a] (ab50852) at 1/50 dilution + A2058 whole cell lysate at 50 µg
Mouse IgG antibody
Predicted band size: 296 kDa
Observed band size: >250 kDa why is the actual band size different from the predicted?
ab50852 has been referenced in 2 publications.
- Cucco F et al. Pathogenic variants in EP300 and ANKRD11 in patients with phenotypes overlapping Cornelia de Lange syndrome. Am J Med Genet A 182:1690-1696 (2020). PubMed: 32476269
- Garee JP et al. Regulation of HER2 oncogene transcription by a multifunctional coactivator/corepressor complex. Mol Endocrinol 28:846-59 (2014). PubMed: 24678732