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RecombinantRabMAb

Recombinant Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)

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Immunocytochemistry/ Immunofluorescence - Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
  • Western blot - Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
  • Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10730(B)] to ANKRD2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-ANKRD2 antibody [EPR10730(B)]
    See all ANKRD2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10730(B)] to ANKRD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ANKRD2. The exact sequence is proprietary.

  • Positive control

    • HeLa cells. Human fetal muscle, A673, HT-1080 and HeLa cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10730(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types

Associated products

  • Alternative Versions

    • Anti-ANKRD2 antibody [EPR10730(B)] - BSA and Azide free (ab249287)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF
1/50 - 1/100.

Target

  • Relevance

    ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs are induced in response to physiologic stress, injury, and hypertrophy. ANKRD2 may play an important role in skeletal muscle hypertrophy.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 26287 Human
    • Omim: 610734 Human
    • SwissProt: Q9GZV1 Human
    • Unigene: 73708 Human
    • Alternative names

      • Ankyrin repeat domain 2 antibody
      • Ankyrin repeat domain 2 stretch responsive muscle antibody
      • Ankyrin repeat domain containing protein 2 antibody
      • Ankyrin repeat protein antibody
      • Ankyrin repeat protein with PEST motif and proline-rich region antibody
      • ARPP antibody
      • hArpp antibody
      • Skeletal muscle ankyrin repeat protein antibody
      • Weakly similar to cytokine inducible nuclear protein C193 H sapiens antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
      Immunocytochemistry/ Immunofluorescence - Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
      Immunofluorescent analysis of Hela cells labeling ANKRD2 with ab156875 at 1/50 dilution.
    • Western blot - Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
      Western blot - Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
      All lanes : Anti-ANKRD2 antibody [EPR10730(B)] (ab156875) at 1/1000 dilution

      Lane 1 : Human fetal muscle lysates
      Lane 2 : A673 cell lysates
      Lane 3 : HT-1080 cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    • Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)
      Anti-ANKRD2 antibody [EPR10730(B)] (ab156875)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab156875? Please let us know so that we can cite the reference in this datasheet.

    ab156875 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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