Anti-ANKRD23 antibody (ab122320)
Key features and details
- Rabbit polyclonal to ANKRD23
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ANKRD23 antibody -
Description
Rabbit polyclonal to ANKRD23 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: KRLRHRVPPR KPEPLVKPQS QAQVEPVGLE MFLKAAAENQ EYLIDKYLTD GGDPNAHDKL HRTALHWAC, corresponding to amino acids 84-152 of Human ANKRD23 (Q86SG2).
-
Positive control
- ICC: A-431 cells. IHC: human smooth muscle and prostate tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
-
Function
May be involved in the energy metabolism. Could be a molecular link between myofibrillar stretch-induced signaling pathways and muscle gene expression. -
Tissue specificity
Mainly expressed in heart, skeletal muscle and brown adipose tissues. -
Sequence similarities
Contains 4 ANK repeats. -
Cellular localization
Nucleus. Sarcomeric I-band and some intercalated disks. - Information by UniProt
-
Database links
- Entrez Gene: 200539 Human
- Omim: 610736 Human
- SwissProt: Q86SG2 Human
- Unigene: 643430 Human
-
Alternative names
- ANKRD23 antibody
- Ankyrin repeat domain 23 antibody
- Ankyrin repeat domain-containing protein 23 antibody
see all
Images
-
Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human prostate with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells
-
All lanes : Anti-ANKRD23 antibody (ab122320) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) -
Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization: PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab122320 has been referenced in 1 publication.
- Wette SG et al. Characterization of muscle ankyrin repeat proteins in human skeletal muscle. Am J Physiol Cell Physiol 313:C327-C339 (2017). PubMed: 28615162