Key features and details
- Rabbit polyclonal to ANKRD23
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKRD23 antibody
See all ANKRD23 primary antibodies
DescriptionRabbit polyclonal to ANKRD23
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- ICC: A-431 cells. IHC: human smooth muscle and prostate tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionMay be involved in the energy metabolism. Could be a molecular link between myofibrillar stretch-induced signaling pathways and muscle gene expression.
Tissue specificityMainly expressed in heart, skeletal muscle and brown adipose tissues.
Sequence similaritiesContains 4 ANK repeats.
Cellular localizationNucleus. Sarcomeric I-band and some intercalated disks.
- Information by UniProt
- ANKRD23 antibody
- Ankyrin repeat domain 23 antibody
- Ankyrin repeat domain-containing protein 23 antibody
Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human prostate with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells
All lanes : Anti-ANKRD23 antibody (ab122320) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization: PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.
ab122320 has been referenced in 1 publication.
- Wette SG et al. Characterization of muscle ankyrin repeat proteins in human skeletal muscle. Am J Physiol Cell Physiol 313:C327-C339 (2017). PubMed: 28615162