Anti-Ankrd26 antibody (ab47984)

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Western blot - Anti-Ankrd26 antibody (ab47984)

    Key features and details

    • Rabbit polyclonal to Ankrd26
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Ankrd26 antibody
      See all Ankrd26 primary antibodies

    • Description

      Rabbit polyclonal to Ankrd26

    • Host species

      Rabbit

    • Specificity

      This antibody is specific for mouse Ankrd26.

    • Tested Applications & Species

      Application Species
      WB
      Human
      Recombinant fragment
      See all applications and species data

    • Immunogen

      Synthetic peptide:

      MKKIFGFRSKGPSPL

      , corresponding to amino acids 1-15 of Mouse Ankrd26
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • MES cell lysate

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab47984 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Recombinant fragment
    All applications
    Rat
    Cow
    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 196 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 196 kDa.

    Target

    Images

    • Western blot - Anti-Ankrd26 antibody (ab47984)
      Western blot - Anti-Ankrd26 antibody (ab47984)
      Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution

      Lane 1 : BLANK
      Lane 2 : MES cell lysate - 80µg
      Lane 3 : MES cell lysate - 40µg
      Lane 4 : 293T-ANKRD26
      transfected cell lysate - 20µg
      Lane 5 : control 293T cell
      lysate - 20µg
      Lane 6 : BSA-ANKRD26 conjugate - 20ng
      Lane 7 : BSA - 500ng
      Lane 8 : BSA - 100ng
      Lane 9 : BSA - 20ng
      Lane 10 : Protein standards

      Secondary
      Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution

      Predicted band size: 196 kDa
      Observed band size: 81 kDa why is the actual band size different from the predicted?



      Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.

    References (1)

    Publishing research using ab47984? Please let us know so that we can cite the reference in this datasheet.

    ab47984 has been referenced in 1 publication.

    • Cardamone MD  et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . PubMed: 24953653

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