Anti-Ankrd26 antibody (ab47984)

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Overview

  • Product name

    Anti-Ankrd26 antibody
    See all Ankrd26 primary antibodies

  • Description

    Rabbit polyclonal to Ankrd26

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for mouse Ankrd26.

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cow

  • Immunogen

    Synthetic peptide:

    MKKIFGFRSKGPSPL

    , corresponding to amino acids 1-15 of Mouse Ankrd26

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • MES cell lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/80000.
WB 1/500 - 1/3000. Predicted molecular weight: 196 kDa.

Target

Images

  • Western blot - Anti-Ankrd26 antibody (ab47984)
    Western blot - Anti-Ankrd26 antibody (ab47984)
    Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution

    Lane 1 : BLANK
    Lane 2 : MES cell lysate - 80µg
    Lane 3 : MES cell lysate - 40µg
    Lane 4 : 293T-ANKRD26
    transfected cell lysate - 20µg
    Lane 5 : control 293T cell
    lysate - 20µg
    Lane 6 : BSA-ANKRD26 conjugate - 20ng
    Lane 7 : BSA - 500ng
    Lane 8 : BSA - 100ng
    Lane 9 : BSA - 20ng
    Lane 10 : Protein standards

    Secondary
    Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution

    Predicted band size: 196 kDa
    Observed band size: 81 kDa
    why is the actual band size different from the predicted?



    Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.

References

This product has been referenced in:

  • Cardamone MD  et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . Read more (PubMed: 24953653) »
See 1 Publication for this product

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