Anti-Ankrd26 antibody (ab47984)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Ankrd26 antibody
See all Ankrd26 primary antibodies -
DescriptionRabbit polyclonal to Ankrd26
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Host speciesRabbit
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SpecificityThis antibody is specific for mouse Ankrd26.
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide:
MKKIFGFRSKGPSPL
, corresponding to amino acids 1-15 of Mouse Ankrd26 -
Positive control
- MES cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab47984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/20000 - 1/80000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 196 kDa. |
Target
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Sequence similaritiesContains 5 ANK repeats.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 526041 Cow
- Entrez Gene: 232339 Mouse
- Entrez Gene: 312667 Rat
- SwissProt: Q811D2 Mouse
- Unigene: 276116 Mouse
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Alternative names
- Ankrd26 antibody
- Ankyrin repeat domain 26 antibody
- Ankyrin repeat domain-containing protein 26 antibody
see all
Images
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Western blot - Anti-Ankrd26 antibody (ab47984)Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution
Lane 1 : BLANK
Lane 2 : MES cell lysate - 80µg
Lane 3 : MES cell lysate - 40µg
Lane 4 : 293T-ANKRD26
transfected cell lysate - 20µg
Lane 5 : control 293T cell
lysate - 20µg
Lane 6 : BSA-ANKRD26 conjugate - 20ng
Lane 7 : BSA - 500ng
Lane 8 : BSA - 100ng
Lane 9 : BSA - 20ng
Lane 10 : Protein standards
Secondary
Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution
Predicted band size: 196 kDa
Observed band size: 81 kDa (why is the actual band size different from the predicted?)
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.
Protocols
References
This product has been referenced in:
- Cardamone MD et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . Read more (PubMed: 24953653) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"