Product nameAnti-Ankrd26 antibody
See all Ankrd26 primary antibodies
DescriptionRabbit polyclonal to Ankrd26
SpecificityThis antibody is specific for mouse Ankrd26.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow
- MES cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab47984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/80000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 196 kDa.|
Sequence similaritiesContains 5 ANK repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Ankrd26 antibody
- Ankyrin repeat domain 26 antibody
- Ankyrin repeat domain-containing protein 26 antibody
Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution
Lane 1 : BLANK
Lane 2 : MES cell lysate - 80µg
Lane 3 : MES cell lysate - 40µg
Lane 4 : 293T-ANKRD26
transfected cell lysate - 20µg
Lane 5 : control 293T cell
lysate - 20µg
Lane 6 : BSA-ANKRD26 conjugate - 20ng
Lane 7 : BSA - 500ng
Lane 8 : BSA - 100ng
Lane 9 : BSA - 20ng
Lane 10 : Protein standards
Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution
Predicted band size: 196 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.