Anti-Ankrd26 antibody (ab47984)
Key features and details
- Rabbit polyclonal to Ankrd26
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ankrd26 antibody
See all Ankrd26 primary antibodies -
Description
Rabbit polyclonal to Ankrd26 -
Host species
Rabbit -
Specificity
This antibody is specific for mouse Ankrd26. -
Tested Applications & Species
Application Species WB HumanRecombinant fragment -
Immunogen
Synthetic peptide:
MKKIFGFRSKGPSPL
, corresponding to amino acids 1-15 of Mouse Ankrd26 -
Positive control
- MES cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
Recombinant fragment
|
All applications |
Rat
Cow
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Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 196 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 196 kDa. |
Target
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Sequence similarities
Contains 5 ANK repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 526041 Cow
- Entrez Gene: 22852 Human
- Entrez Gene: 312667 Rat
- Omim: 610855 Human
- SwissProt: Q9UPS8 Human
- Unigene: 361041 Human
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Alternative names
- Ankrd26 antibody
- Ankyrin repeat domain 26 antibody
- Ankyrin repeat domain-containing protein 26 antibody
see all
Images
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Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution
Lane 1 : BLANK
Lane 2 : MES cell lysate - 80µg
Lane 3 : MES cell lysate - 40µg
Lane 4 : 293T-ANKRD26
transfected cell lysate - 20µg
Lane 5 : control 293T cell
lysate - 20µg
Lane 6 : BSA-ANKRD26 conjugate - 20ng
Lane 7 : BSA - 500ng
Lane 8 : BSA - 100ng
Lane 9 : BSA - 20ng
Lane 10 : Protein standards
Secondary
Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution
Predicted band size: 196 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab47984 has been referenced in 1 publication.
- Cardamone MD et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . PubMed: 24953653