Overview

  • Product name
  • Description
    Rabbit polyclonal to Ankrd26
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for mouse Ankrd26.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    MKKIFGFRSKGPSPL

    , corresponding to amino acids 1-15 of Mouse Ankrd26

  • Positive control
    • MES cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/80000.
WB 1/500 - 1/3000. Predicted molecular weight: 196 kDa.

Target

Images

  • Lanes 2-10 : Anti-Ankrd26 antibody (ab47984) at 1/1000 dilution

    Lane 1 : BLANK
    Lane 2 : MES cell lysate - 80µg
    Lane 3 : MES cell lysate - 40µg
    Lane 4 : 293T-ANKRD26
    transfected cell lysate - 20µg
    Lane 5 : control 293T cell
    lysate - 20µg
    Lane 6 : BSA-ANKRD26 conjugate - 20ng
    Lane 7 : BSA - 500ng
    Lane 8 : BSA - 100ng
    Lane 9 : BSA - 20ng
    Lane 10 : Protein standards

    Secondary
    Lanes 2-10 : ALP conjugated Gt-a-Rabbit IgG at 1/3000 dilution

    Predicted band size: 196 kDa
    Observed band size: 81 kDa
    why is the actual band size different from the predicted?



    Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. An observed band of ~81kDa has been obtained due to a fragment of Ankrd26 being used instead of the full length version.

References

This product has been referenced in:
  • Cardamone MD  et al. GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPAR?. Cell Rep 8:163-76 (2014). Mouse . Read more (PubMed: 24953653) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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