Key features and details
- Rabbit polyclonal to ANKRD35
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKRD35 antibody
DescriptionRabbit polyclonal to ANKRD35
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- RT-4 and U251-MG cell lysates; Human tonsil lysate; Human nasopharynx tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 6 ANK repeats.
- Information by UniProt
- ANKRD35 antibody
- Ankyrin repeat domain 35 antibody
- Ankyrin repeat domain-containing protein 35 antibody
- ANR35_HUMAN antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122279 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-ANKRD35 antibody (ab122279) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U251-MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab122279, at 1/250 dilution, staining ANKRD35 in paraffin-embedded Human nasopharynx tissue by Immunohistochemistry.
ab122279 has not yet been referenced specifically in any publications.