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Rabbit polyclonal to ANKRD50
Predicted to work with:
Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Elephant
Synthetic peptide corresponding to a region between residue 1379 and 1429 of Human ANKRD50 (NP_065070.1).
HeLa, 293T and NIH 3T3 whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab108219 was affinity purified using an epitope specific to ANKRD50 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 156 kDa.
Use at 2-5 µg/mg of lysate.
Contains 19 ANK repeats.
Information by UniProt
Ankyrin repeat domain 50 antibody
Ankyrin repeat domain-containing protein 50 antibody
Western blot - Anti-ANKRD50 antibody (ab108219)
All lanes : Anti-ANKRD50 antibody (ab108219) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 156 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-ANKRD50 antibody (ab108219)
ab108219 at 1 µg/ml detecting ANKRD50 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of ANKRD50
Lane 2: ab108219 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"