Product nameAnti-ANKRD57 antibody
See all ANKRD57 primary antibodies
DescriptionRabbit polyclonal to ANKRD57
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ANKRD57 aa 475-525. The exact sequence is proprietary.
Database link: Q53LP3
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Our Abpromise guarantee covers the use of ab99381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Sequence similaritiesBelongs to the SOWAH family.
Contains 2 ANK repeats.
- Information by UniProt
- 4921515A04Rik antibody
- 9430099J10Rik antibody
- Aankyrin repeat domain 5 antibody
All lanes : Anti-ANKRD57 antibody (ab99381) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 56 kDa
Exposure time: 30 seconds
ANKRD57 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab99381 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab99381 at 0.1 µg/ml.
Lane 1: ab99381 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab99381 has not yet been referenced specifically in any publications.