Key features and details
- Rabbit polyclonal to ANKS1A
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKS1A antibody
DescriptionRabbit polyclonal to ANKS1A
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human testis, Fallopian tube and colon tissue. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a negative role in growth factor receptor signaling pathways.
Tissue specificityWidely expressed (at protein level).
Sequence similaritiesContains 6 ANK repeats.
Contains 1 PID domain.
Contains 2 SAM (sterile alpha motif) domains.
modificationsPhosphorylated on tyrosine residues in response to EGF and PDGF.
Cellular localizationCytoplasm. Cytoplasmic before and after growth factor treatment.
- Information by UniProt
- ANKS 1 antibody
- ANKS 1A antibody
- ANKS1 antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ANKS1A using ab254690 at 4µg/ml (green) in ICC/IF.
Paraffin-embedded human testis tissue stained for ANKS1A using ab254690 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human Fallopian tube tissue stained for ANKS1A with ab254690 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for ANKS1A with ab254690 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue shows absence of immunoreactivity for ANKS1A when incubated with ab254690 at 1/500 dilution (negative control).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254690 has not yet been referenced specifically in any publications.