Key features and details
- Rabbit polyclonal to ANKS1B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKS1B antibody
DescriptionRabbit polyclonal to ANKS1B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- NCI-H292 cell line lysate; Human liver and kidney tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 138 kDa.|
FunctionIsoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.
Isoform 3 can regulate global protein synthesis by altering nucleolar numbers.
Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing.
Tissue specificityHighly expressed in marrow from patients with pre-B ALL associated with the t(1;19) translocation. Strongly expressed in brain and testis. Expressed in fetal brain. Isoform 4 is highly expressed in brain (at protein level). Isoform 6 is expressed in brain and several cancer cell lines.
Sequence similaritiesContains 7 ANK repeats.
Contains 1 PID domain.
Contains 2 SAM (sterile alpha motif) domains.
modificationsIsoform 3 nuclear translocation requires an NMDAR-dependent proteolytic cleavage.
Cellular localizationCytoplasm; Nucleus; Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendritic spine. Nucleus. Nucleus > Cajal body. The synaptic localization requires DLG4 interaction. Translocation to the nucleus in response to stimulation of NMDA receptors (NMDARs) in a calcium-independent manner and Nucleus. The interaction with APP causes its partial exclusion from the nucleus, when APP is overexpressed.
- Information by UniProt
- AIDA antibody
- AIDA-1 antibody
- Amyloid-beta protein intracellular domain-associated protein 1 antibody
Anti-ANKS1B antibody (ab192658) at 1/1000 dilution + NCI-H292 cell line lysate at 35 µg
Predicted band size: 138 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling ANKS1B with ab192658 at 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ANKS1B with ab192658 at 1/100 dilution.
ab192658 has not yet been referenced specifically in any publications.