Western blot abreview for Anti-ANKS1B antibody - C-terminal

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Abreviews
Application
Western blot
Sample
Rat Tissue lysate - other (Neurons-Synaptosomes)
Loading amount
30 µg
Specification
Neurons-Synaptosomes
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Concentration
2 µg/ml
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS+ 0.05% tween-20

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Positive control
Synaptosomes isolated from brain tissues in wildtype mice. Western blots were also performed using another ANKS1b antibody known to work (AIDA-1 m1B5).
Negative control
Synaptosomes isolated from brain tissues from ANKS1b(AIDA-1) knockout mice

Additional data

Additional Notes
Neither Immunoprecipitations nor immunofluorescene of rat neurons revealed any detectable signal, which is confirmed by no signal observed in western blots.
Abcam response
Thank you for your valuable feedback. This antibody has not been tested in rat and may not be suitable for use in this species.

Mr. Bryen A. Jordan

Verified customer

Submitted Mar 11 2013

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