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Rat Tissue lysate - other (Neurons-Synaptosomes)
Gel Running Conditions
Reduced Denaturing (10%)
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
2 hour(s) and 0 minute(s) · Diluent: TBS+ 0.05% tween-20
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Goat
Conjugation: Horse Radish Peroxidase
5 minute(s) and 0 second(s)
Synaptosomes isolated from brain tissues in wildtype mice. Western blots were also performed using another ANKS1b antibody known to work (AIDA-1 m1B5).
Synaptosomes isolated from brain tissues from ANKS1b(AIDA-1) knockout mice
Neither Immunoprecipitations nor immunofluorescene of rat neurons revealed any detectable signal, which is confirmed by no signal observed in western blots.
Thank you for your valuable feedback. This antibody has not been tested in rat and may not be suitable for use in this species.
Mr. Bryen A. Jordan
Submitted Mar 11 2013