Overview

  • Product name

    Anti-Ankyrin brain antibody [N105/13]
  • Description

    Mouse monoclonal [N105/13] to Ankyrin brain
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against Ankyrin-G
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 203-496 of Human Ankyrin brain.

  • Positive control

    • WB: Rat brain lysate. Flow Cyt: SH-SY5Y cells. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S105-13 to N105/13, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab131419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 430 kDa). 1 µg/mL of ab131419 was sufficient for detection of Ankyrin brain in 20µg of Rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
IHC-Fr 1/50 - 1/200.
IHC-P 1/50 - 1/200.
ICC/IF 1/200.

Target

  • Function

    Attaches integral membrane proteins to cytoskeletal elements. Also binds to cytoskeletal proteins. Required for coordinate assembly of Na/Ca exchanger, Na/K ATPase and InsP3 receptor at sarcoplasmic reticulum sites in cardiomyocytes. Required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) in the inner segment of rod photoreceptors. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate.
  • Tissue specificity

    Present in plasma membrane of neurons as well as glial cells throughout the brain. Expressed in fetal brain and in temporal cortex of adult brain. Also expressed in the inner segments of rod photoreceptors in retina.
  • Involvement in disease

    Defects in ANK2 are the cause of long QT syndrome type 4 (LQT4) [MIM:600919]; also known as sick sinus syndrome with bradycardia. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. LQT4 displays many atypical features compared to classical long QT syndromes, including pronounced sinus bradycardia, polyphasic T waves and atrial fibrillation. Cardiac repolarization defects may be not as severe as in classical LQT syndromes and prolonged QT interval on EKG is not a consistent feature.
  • Sequence similarities

    Contains 24 ANK repeats.
    Contains 1 death domain.
    Contains 1 ZU5 domain.
  • Post-translational
    modifications

    Phosphorylated at multiple sites by different protein kinases and each phosphorylation event regulates the protein's structure and function.
  • Cellular localization

    Cytoplasm > cytoskeleton. Membrane. Cytoplasm > myofibril > sarcomere > M line. Apical cell membrane. Cell membrane. Expressed at the apical membrane of airway lung epithelial cells (By similarity). Localized to the plasma membrane of the inner segments of photoreceptors in retina. Colocalizes with SPTBN1 in a distict intracellular compartment of neonatal cardiomyocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANK-2 antibody
    • Ank2 antibody
    • ANK2_HUMAN antibody
    • Ankyrin 2 antibody
    • Ankyrin 2 brain antibody
    • Ankyrin B antibody
    • Ankyrin nonerythroid antibody
    • ANKYRIN, NEURONAL antibody
    • Ankyrin-2 antibody
    • Ankyrin-B antibody
    • Brain ankyrin antibody
    • Non-erythroid ankyrin antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for Ankyrin brain (green) using ab131419 at 1/100 dilution in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.

  • Overlay histogram showing SH-SY5Y cells stained with ab131419 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131419, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot detection of Ankyrin brain in Rat brain membrane tissues using ab131419 at 1:1000 dilution.

References

This product has been referenced in:

  • Cao W  et al. Regulation of drug resistance and metastasis of gastric cancer cells via the microRNA647-ANK2 axis. Int J Mol Med 41:1958-1966 (2018). Read more (PubMed: 29328428) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens haben wir noch keine Abbildung von ab104896 und ab131419 in IHC-P und WB.
Falls Sie uns eine Abbildung Ihrer Ergebnisse mit ab104896 und ab131419 zukommen lassen, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat und einer Abbildung zusenden. Der Rabatt würde den ganzen Wert von ab104896 und ab131419 abdecken, und gegen eine erneute Bestellung weitere Produkte von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab104896 und ab131419 kaufen möchten und bereit sind uns eine Abbildung Ihrer Ergebnisse zukommen zu lassen.

2.) Bitte bestellen Sie erst nach Erhalt der Rabattcodes.

3.) Bestellen Sie die Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie die Antikörper in IHC-P und/oder WB.

5.) Senden Sie uns die Abbildung Ihres Ergebnisses mittels eines Abreviews zu und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können weitere Produkte zu dem gleichen Preis wie ab104896 und ab131419 bei uns bestellen. Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie Rabattcodes erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up