Product nameAnti-Ankyrin erythroid / ANK antibody
See all Ankyrin erythroid / ANK primary antibodies
DescriptionRabbit polyclonal to Ankyrin erythroid / ANK
SpecificityThe 14 AA immnunogen for this antibody is present in isoform Er5, 6, 11, 12, 15, Mu17, Mu18 and Mu20, P16157. Mu17, 18 and 20 are 17 kd, 15 kd and 8 kd respectively. The antibody preferentially detects Mu17 and we are not sure whether it can detect other isoforms.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to Human Ankyrin erythroid/ ANK (C terminal). The immunogen is a 14aa peptide within this sequence: RRQIDLSSAD AAQEHEEVEL RGSGLQPDLI EGRKGAQIVK RASLKRGKQ, corresponding to C terminal amino acids of human Ankyrin erythroid/ ANK.
- Jurkat whole cell lysate (ab7899) Squamous epithelial cells of human skin Alveolar cells of human lung
Protein previously labeled as Ankyrin erythroid.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionAttaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.
Isoform Mu17 together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
Tissue specificityIsoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.
Involvement in diseaseDefects in ANK1 are a cause of spherocytosis type 1 (SPH1) [MIM:182900]; also called hereditary spherocytosis type 1 (HS1). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Inheritance can be autosomal dominant or recessive.
Sequence similaritiesContains 23 ANK repeats.
Contains 1 death domain.
Contains 1 ZU5 domain.
DomainThe 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.
The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.
modificationsRegulated by phosphorylation.
Cellular localizationCytoplasm > cytoskeleton. Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane; Sarcoplasmic reticulum and Membrane. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.
- Information by UniProt
- ANK antibody
- ANK-1 antibody
- Ank1 antibody
All lanes : Anti-Ankyrin erythroid / ANK antibody (ab58698) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Jurkat cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Gel concentration: 15%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Ankyrin erythroid / ANK with ab58698 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Ankyrin erythroid / ANK with ab58698 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
This product has been referenced in:
- Hall AE et al. The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration. Cell Death Dis 7:e2184 (2016). WB . Read more (PubMed: 27054339) »
- Yan R et al. A novel type 2 diabetes risk allele increases the promoter activity of the muscle-specific small ankyrin 1 gene. Sci Rep 6:25105 (2016). Read more (PubMed: 27121283) »