Key features and details
- Mouse monoclonal [S388A-10] to Ankyrin erythroid/ANK
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2b
Product nameAnti-Ankyrin erythroid/ANK antibody [S388A-10]
DescriptionMouse monoclonal [S388A-10] to Ankyrin erythroid/ANK
Does not cross-react with Ankyrin-B or Ankyrin-G.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Fusion protein corresponding to Human Ankyrin erythroid/ANK aa 1-1881.
Database link: P16157
- WB: Rat brain tissue lysate. ICC/IF: SK-N-BE cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab212053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 206 kDa.|
FunctionAttaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.
Isoform Mu17 together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
Tissue specificityIsoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.
Involvement in diseaseDefects in ANK1 are a cause of spherocytosis type 1 (SPH1) [MIM:182900]; also called hereditary spherocytosis type 1 (HS1). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Inheritance can be autosomal dominant or recessive.
Sequence similaritiesContains 23 ANK repeats.
Contains 1 death domain.
Contains 1 ZU5 domain.
DomainThe 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.
The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.
modificationsRegulated by phosphorylation.
Cellular localizationCytoplasm > cytoskeleton. Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane; Sarcoplasmic reticulum and Membrane. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.
- Information by UniProt
- ANK antibody
- ANK-1 antibody
- Ank1 antibody
Anti-Ankyrin erythroid/ANK antibody [S388A-10] (ab212053) at 1/1000 dilution + Rat brain tissue lysate at 20 µg
Goat Anti-Mouse IgG: HRP at 1/200 dilution
Predicted band size: 206 kDa
Primary incubation 16 hours at 4°C.
4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Ankyrin erythroid/ANK (green, C) using ab212053 at 1/100 dilution in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab212053 has not yet been referenced specifically in any publications.