Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604)
Key features and details
- Rabbit polyclonal to Ankyrin repeat domain-containing protein 65
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ankyrin repeat domain-containing protein 65 antibody -
Description
Rabbit polyclonal to Ankyrin repeat domain-containing protein 65 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Ankyrin repeat domain-containing protein 65 aa 1-100.
Database link: E5RJM6 -
Positive control
- WB: RT-4 and U-251 MG whole cell lysates; ICC: RT4 whole cell lysates; IHC-P: Human Fallopian tube tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 10 ANK repeats. - Information by UniProt
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Database links
- Entrez Gene: 441869 Human
- SwissProt: E5RJM6 Human
- Unigene: 381222 Human
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Alternative names
- ANKRD65 antibody
- Ankyrin repeat domain-containing protein 65 antibody
- ANR65_HUMAN antibody
see all
Images
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Immunohistochemical analysis of human Fallopian tube tissue labeling Ankyrin repeat domain-containing protein 65 (high expression) with ab204604 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of RT4 (Human urinary bladder cancer cell line) whole cells labeling Ankyrin repeat domain-containing protein 65 in the nucleoplasm with ab204604 at 2 µg/ml.
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All lanes : Anti-Ankyrin repeat domain-containing protein 65 antibody (ab204604) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 41 kDa -
Immunohistochemical analysis of human kidney tissue labeling Ankyrin repeat domain-containing protein 65 (low expression) with ab204604 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204604 has not yet been referenced specifically in any publications.