Key features and details
- Rabbit polyclonal to Annexin-11/ANXA11
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Annexin-11/ANXA11 antibody
See all Annexin-11/ANXA11 primary antibodies
DescriptionRabbit polyclonal to Annexin-11/ANXA11
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Annexin-11/ANXA11 aa 1-299.
- Recombinant Human Annexin-11/ANXA11 protein (ab101050) can be used as a positive control in WB. A431 whole cell lysate; HeLa cells.
This product was previously labelled as Annexin A11
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionBinds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
Cellular localizationCytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent.
- Information by UniProt
- 56 kDa autoantigen antibody
- Annexin A11 antibody
- Annexin XI antibody
Anti-Annexin-11/ANXA11 antibody (ab137424) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Annexin-11/ANXA11 with ab137424 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
ab137424 has been referenced in 1 publication.
- Chang YT et al. Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis. Am J Cancer Res 10:12-37 (2020). PubMed: 32064151