Key features and details
- Rabbit polyclonal to Annexin-11/ANXA11
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Annexin-11/ANXA11 antibody
See all Annexin-11/ANXA11 primary antibodies
DescriptionRabbit polyclonal to Annexin-11/ANXA11
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
Recombinant fragment corresponding to Human Annexin-11/ANXA11 aa 276-505.
FDIYEIKEAIKGVGTDEACLIEILASRSNEHIRELNRAYKAEFKKTLEEA IRSDTSGHFQRLLISLSQGNRDESTNVDMSLAQRDAQELYAAGENRLGTD ESKFNAVLCSRSRAHLVAVFNEYQRMTGRDIEKSICREMSGDLEEGMLAV VKCLKNTPAFFAERLNKAMRGAGTKDRTLIRIMVSRSETDLLDIRSEYKR MYGKSLYHDISGDTSGDYRKILLKICGGND
Database link: P50995
- WB: U-251 MG cell lysate. IHC-P: Human kidney and placenta tissue. ICC/IF: HeLa cells.
This product was previously labelled as Annexin A11
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab236599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000.|
|ICC/IF||1/50 - 1/200.|
FunctionBinds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
Cellular localizationCytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent.
- Information by UniProt
- 56 kDa autoantigen antibody
- Annexin A11 antibody
- Annexin XI antibody
Anti-Annexin-11/ANXA11 antibody (ab236599) at 1/500 dilution + U-251 MG (human brain glioma cell line) cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Paraffin-embedded human kidney tissue stained for Annexin-11/ANXA11 using ab236599 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for Annexin-11/ANXA11 using ab236599 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Annexin-11/ANXA11 using ab236599 at 1/100 dilution in ICC/IF. Secondary antibody was Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236599 has not yet been referenced specifically in any publications.