Key features and details
- Goat polyclonal to Annexin-11/ANXA11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Annexin-11/ANXA11 antibody
See all Annexin-11/ANXA11 primary antibodies
DescriptionGoat polyclonal to Annexin-11/ANXA11
SpecificityThis antibody is expected to recognise all three human isoforms.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- HeLa lysate.
This product was previously labelled as Annexin A11
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
A 1 hour primary incubation is recommended for this product. Approx 55 kDa band seen in HeLa lysate (Predicted MW of approx. 58 kDa according to NP_001148).
FunctionBinds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
Cellular localizationCytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent.
- Information by UniProt
- 56 kDa autoantigen antibody
- Annexin A11 antibody
- Annexin XI antibody
All lanes : Anti-Annexin-11/ANXA11 antibody (ab4121) at 0.01 µg/ml
Lane 1 : HeLa lysates (in RIPA buffer)
Lane 2 : Jurkat lysates (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Detected by chemiluminescence.
ab4121 has not yet been referenced specifically in any publications.