Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody
    See all Annexin-2/ANXA2 primary antibodies
  • Description

    Rabbit polyclonal to Annexin-2/ANXA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Annexin-2/ANXA2 aa 1-46 (N terminal).

  • Positive control

    • WB: HeLa, A549 and HCT 116 whole cell lysates. IHC-P: Human pancreas tissue. ICC/IF: HeLa cells.
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as Annexin A2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab137645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.

Target

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links

  • Alternative names

    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Annexin-2/ANXA2 knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 40 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labelling Annexin-2/ANXA2 with ab137645(Green) at 1/500 dilution. Alpha tubulin filaments were labeled with alpha tubulin antibody (Red) at 1/2000.

  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution

    Lane 1 : Untransfected HEK-293T whole cell extract
    Lane 2 : Annexin 1 transfected HEK-293T whole cell extract
    Lane 3 : Annexin 2 transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 40 kDa

  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab137645) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate/extract
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 40 kDa

  • Immunohistochemical analysis of paraffin embedded Human pancreas tissue, labelling Annexin-2/ANXA2 with ab137645 at 1/100 dilution: X20 magnification (left), X40 magnification (right)

References

ab137645 has not yet been referenced specifically in any publications.

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