Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody
    See all Annexin-2/ANXA2 primary antibodies
  • Description

    Rabbit polyclonal to Annexin-2/ANXA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Annexin-2/ANXA2 aa 19-237.
    Database link: P07355

  • Positive control

    • A549, HCT116, GL261, NIH 3T3, C2C12 and Rat2 whole cell lysates; A431 cells; Human colon cancer tissue
  • General notes

     This product was previously labelled as Annexin A2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links

  • Alternative names

    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Annexin-2/ANXA2 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 38 kDa

  • Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution + Rat2 whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

    Lane 1 : GL261 whole cell lysate
    Lane 2 : NIH 3T3 whole cell lysate
    Lane 3 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10% SDS PAGE
  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa

  • Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Annexin-2/ANXA2 with ab154113 at 1/200 dilution.
    The image in the lower panel was merged with DNA probe.

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Annexin-2/ANXA2 with ab154113 at 1/500 dilution.

References

ab154113 has not yet been referenced specifically in any publications.

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