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    annexin-2anxa2-antibody-ab41803.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Annexins
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Anti-Annexin-2/ANXA2 antibody (ab41803)

  • Datasheet
  • SDS
Reviews (16)Q&A (2)References (60)

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Western blot - Anti-Annexin-2/ANXA2 antibody (ab41803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab41803)
  • Immunocytochemistry - Anti-Annexin-2/ANXA2 antibody (ab41803)
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab41803)
  • Western blot - Anti-Annexin-2/ANXA2 antibody (ab41803)

Key features and details

  • Rabbit polyclonal to Annexin-2/ANXA2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody
    See all Annexin-2/ANXA2 primary antibodies
  • Description

    Rabbit polyclonal to Annexin-2/ANXA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human Annexin-2/ANXA2 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41802)

  • Positive control

    • WB: HeLa, HEK293, HepG2, MCF7 and SHSY-5Y whole cell lysates. ICC/IF: HeLa and MCF7 cells. IHC-P: Human breast carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Annexins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Annexin-2/ANXA2 protein (ab93005)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (5)
Use a concentration of 1 - 5 µg/ml.
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P (6)
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Target information above from: UniProt accession P07355 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 302 Human
    • Entrez Gene: 12306 Mouse
    • Entrez Gene: 56611 Rat
    • Omim: 151740 Human
    • SwissProt: P07355 Human
    • SwissProt: P07356 Mouse
    • SwissProt: Q07936 Rat
    • Unigene: 511605 Human
    • Unigene: 238343 Mouse
    • Unigene: 90546 Rat
    see all
  • Alternative names

    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • Western blot - Anti-Annexin-2/ANXA2 antibody (ab41803)
    Western blot - Anti-Annexin-2/ANXA2 antibody (ab41803)
    All lanes : Anti-Annexin-2/ANXA2 antibody (ab41803) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab41803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab41803)

    IHC image of Annexin-2/ANXA2 staining in FFPE human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41803, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Immunocytochemistry - Anti-Annexin-2/ANXA2 antibody (ab41803)
    Immunocytochemistry - Anti-Annexin-2/ANXA2 antibody (ab41803)

    ab41803 staining Annexin-2 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41803 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab41803)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab41803)

    ICC/IF image of ab41803 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41803, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Annexin-2/ANXA2 antibody (ab41803)
    Western blot - Anti-Annexin-2/ANXA2 antibody (ab41803)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (60)

Publishing research using ab41803? Please let us know so that we can cite the reference in this datasheet.

ab41803 has been referenced in 60 publications.

  • Zhang K & Zhang X MiR-146b-3p protects against AR42J cell injury in cerulein-induced acute pancreatitis model through targeting Anxa2. Open Life Sci 16:255-265 (2021). PubMed: 33817317
  • Zhou L  et al. Exosomal LncRNA LINC00659 transferred from cancer-associated fibroblasts promotes colorectal cancer cell progression via miR-342-3p/ANXA2 axis. J Transl Med 19:8 (2021). PubMed: 33407563
  • Li Y  et al. Identification of Adrenomedullin-Induced S-Nitrosylated Proteins in JEG-3 Placental Cells. Reprod Sci N/A:N/A (2021). PubMed: 34462873
  • Agarwal A  et al. Quantitative mass spectrometric analysis of the mouse cerebral cortex after ischemic stroke. PLoS One 15:e0231978 (2020). PubMed: 32315348
  • Loef EJ  et al. Neuroserpin regulates human T cell-T cell interactions and proliferation through inhibition of tissue plasminogen activator. J Leukoc Biol 107:145-158 (2020). PubMed: 31667914
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin-2/ANXA2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Gallbladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER (pH 9.2, 1 mM EDTA and 10mM Tris Base)
Permeabilization
No
Specification
Gallbladder
Blocking step
3% BSA, 5% Horse Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Dr. Jackson Lab

Verified customer

Submitted Jan 14 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin-2/ANXA2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin-2/ANXA2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Nov 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin-2/ANXA2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate and Tris-EDTA
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

MISS. Emily Santrau

Verified customer

Submitted Aug 05 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin A2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Specification
liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
No
Blocking step
Sequential peroxidase (5min) and protein (10min) block (prediluted) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Mr. Antibody Solutions

Verified customer

Submitted Aug 19 2008

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin-2/ANXA2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Specification
liver
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. (These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Mr. Antibody Solutions

Verified customer

Submitted Aug 19 2008

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