Anti-Annexin-2/ANXA2 antibody (ab41803)

Rabbit polyclonal Annexin A2 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Human, African green monkey. Cited in 34 publication(s). Independently reviewed in 11 review(s).

Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody
    See all Annexin-2/ANXA2 primary antibodies
  • Description

    Rabbit polyclonal to Annexin-2/ANXA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
    Predicted to work with: Rat, Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Annexin-2/ANXA2 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41802)

  • Positive control

    • WB: HeLa, HEK293, HepG2, MCF7 and SHSY-5Y whole cell lysates. ICC/IF: HeLa and MCF7 cells. IHC-P: Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as Annexin A2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab41803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin-2/ANXA2 peptide (ab41802).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links

  • Alternative names

    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • All lanes : Anti-Annexin-2/ANXA2 antibody (ab41803) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • IHC image of Annexin-2/ANXA2 staining in FFPE human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41803, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ICC/IF image of ab41803 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41803, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.
  • ICC/IF image of ab41803 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41803, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Nuerzhati Y  et al. Role of the long non-coding RNA-Annexin A2 pseudogene 3/Annexin A2 signaling pathway in biliary atresia-associated hepatic injury. Int J Mol Med 43:739-748 (2019). Read more (PubMed: 30569159) »
  • Luo GF  et al. FOXD3 may be a new cellular target biomarker as a hypermethylation gene in human ovarian cancer. Cancer Cell Int 19:44 (2019). Read more (PubMed: 30858761) »
See all 39 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (hindpaw skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
hindpaw skin
Blocking step
LiCOR blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C

Maral Tajerian

Verified customer

Submitted Aug 26 2015

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (4-20% tris-Glycin gel)
Sample
Human Cell lysate - whole cell (Platelet Activating Factor cancer cell lines)
Specification
Platelet Activating Factor cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 17 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample
Monkey Cell (COS)
Specification
COS
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 02 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Permeabilization
Yes - 0.1% Triton
Specification
HUVEC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 02 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Permeabilization
Yes - 0.1% Triton X100
Specification
HUVEC
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 17 2013

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Monkey Cell (cos-7)
Specification
cos-7
Permeabilization
Yes - 0.1% Triton X100
Fixative
Paraformaldehyde

Mrs. ice cool

Verified customer

Submitted Sep 17 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 22 2012

Answer

Thank you for your inquiry.

Unfortunately, we did not experimentally and specifically test if the monomer or tetramer form of Annexin A2 are recognized by these antibodies.

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36.

Since both antibodies are suitable for WB and IHC, I assume that the monomer is recognized in reducing denaturing WB and the tetramer is recognized in IHC where the target proteins do not get denatured but fixed in their native state.

I am sorry I could not provide an definitive answer on this occasion and hope this information is nevertheless helpful.

Read More

Answer

Thank you for contacting Abcam.

Forthese antibodies I would recommend using reducing conditions and also initially using a 1/1000 primary antibody dilution, although you may to alter this concentration for subsequent western blots.

If there is anything else I can help you with, please let me know.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Osteoblast cell line)
Loading amount
10 µg
Specification
Osteoblast cell line
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C

Dr. Rodrigo Alves

Verified customer

Submitted Aug 18 2010

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up