Recombinant
RabMAb

Recombinant Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

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Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
    See all Annexin-2/ANXA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Annexin-2/ANXA2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P07355

  • Positive control

    • HeLa, SKBR-3, K562 and HepG2 whole cell lysate (ab7900); Human liver and prostate tissues; HeLa cells and permeabilized K562 cells.

  • General notes

     This product was previously labelled as Annexin A2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.

    • Sequence similarities

      Belongs to the annexin family.
      Contains 4 annexin repeats.

    • Domain

      A pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Post-translational
      modifications

      Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
      ISGylated.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Annexin A2 antibody
      • Annexin II antibody
      • Annexin II, heavy chain antibody
      • Annexin-2 antibody
      • ANX 2 antibody
      • ANX2 antibody
      • ANX2L4 antibody
      • ANXA2 antibody
      • ANXA2_HUMAN antibody
      • arylsulfatase B antibody
      • CAL1H antibody
      • Calpactin I heavy chain antibody
      • calpactin I heavy polypeptide (p36) antibody
      • Calpactin I heavy polypeptide antibody
      • Calpactin-1 heavy chain antibody
      • chromobindin 8 antibody
      • Chromobindin-8 antibody
      • Epididymis secretory protein Li 270 antibody
      • HEL S 270 antibody
      • LIP2 antibody
      • Lipocortin II antibody
      • LPC2 antibody
      • LPC2D antibody
      • p36 antibody
      • P36 protein antibody
      • PAP-IV antibody
      • Placental anticoagulant protein IV antibody
      • Protein I antibody
      see all

    Images

    • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
      Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
      Lanes 1-3 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/20000 dilution
      Lane 4 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa



      Exposure time
      Lanes 1-3: 1 minute
      Lane 4: 3 minutes

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
      Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

      Immunofluorescent analysis of HeLa cells labeling Annexin-2/ANXA2 with ab178677 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Flow Cytometry - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
      Flow Cytometry - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

      Flow cytometric analysis of permeabilized K562 cells labeling Annexin-2/ANXA2 with ab178677 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Pan BL  et al. Effects of MicroRNA-206 on Osteosarcoma Cell Proliferation, Apoptosis, Migration and Invasion by Targeting ANXA2 Through the AKT Signaling Pathway. Cell Physiol Biochem 45:1410-1422 (2018). Read more (PubMed: 29462818) »
    • Koga R  et al. Annexin A2 Mediates the Localization of Measles Virus Matrix Protein at the Plasma Membrane. J Virol 92:N/A (2018). Read more (PubMed: 29491166) »
    See all 4 Publications for this product

    Publishing research using ab178677? Please let us know so that we can cite the reference in this datasheet.

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