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    annexin-2anxa2-antibody-epr13052b-ab178677.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Annexins
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RecombinantRabMAb

Recombinant Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

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Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
  • Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
  • Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

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Overview

  • Product name

    Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
    See all Annexin-2/ANXA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Annexin-2/ANXA2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P07355

  • Positive control

    • HeLa, SKBR-3, K562 and HepG2 whole cell lysate (ab7900); Human liver and prostate tissues; HeLa cells and permeabilized K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13052(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Annexins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200791)
    • Alexa Fluor® 647 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200797)
    • HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200798)
    • PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab210729)
    • Alexa Fluor® 594 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab216386)
    • Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free (ab232476)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human Annexin-2/ANXA2 protein (ab93005)
  • Related Products

    • Recombinant Human Annexin-2/ANXA2 protein (ab93005)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/20000. Predicted molecular weight: 38 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/20000. Predicted molecular weight: 38 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for IP.

Target

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Target information above from: UniProt accession P07355 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 302 Human
    • Entrez Gene: 12306 Mouse
    • Entrez Gene: 56611 Rat
    • Omim: 151740 Human
    • SwissProt: P07355 Human
    • SwissProt: P07356 Mouse
    • SwissProt: Q07936 Rat
    • Unigene: 511605 Human
    • Unigene: 238343 Mouse
    • Unigene: 90546 Rat
    see all
  • Alternative names

    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Lanes 1-3 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/20000 dilution
    Lane 4 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SKBR-3 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa



    Exposure time
    Lanes 1-3: 1 minute
    Lane 4: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

    Immunofluorescent analysis of HeLa cells labeling Annexin-2/ANXA2 with ab178677 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling Annexin-2/ANXA2 with ab178677 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

     

     

  • Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
    Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (16)

Publishing research using ab178677? Please let us know so that we can cite the reference in this datasheet.

ab178677 has been referenced in 16 publications.

  • Hsu CY  et al. Characterization and Proteomic Analysis of Endometrial Stromal Cell-Derived Small Extracellular Vesicles. J Clin Endocrinol Metab 106:1516-1529 (2021). PubMed: 33507273
  • Zhang Y  et al. Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1. Nat Commun 12:1731 (2021). PubMed: 33741962
  • Martínez-Greene JA  et al. Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography. J Extracell Vesicles 10:e12087 (2021). PubMed: 33936570
  • Hernández-Díazcouder A  et al. High fructose exposure modifies the amount of adipocyte-secreted microRNAs into extracellular vesicles in supernatants and plasma. PeerJ 9:e11305 (2021). PubMed: 34055478
  • Shen Y  et al. Multi-Omics Integrative Analysis Uncovers Molecular Subtypes and mRNAs as Therapeutic Targets for Liver Cancer. Front Med (Lausanne) 8:654635 (2021). PubMed: 34109194
View all Publications for this product

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