Recombinant Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
![Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206848-anti-annexin-a2-antibody-epr13052b-western-blot.jpg "Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
See all Annexin-2/ANXA2 primary antibodies -
Description
Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Annexin-2/ANXA2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P07355 -
Positive control
- HeLa, SKBR-3, K562 and HepG2 whole cell lysate (ab7900); Human liver and prostate tissues; HeLa cells and permeabilized K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13052(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200791)
- Alexa Fluor® 647 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200797)
- HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200798)
- PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab210729)
- Alexa Fluor® 594 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab216386)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free (ab232476)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/20000. Predicted molecular weight: 38 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/20000. Predicted molecular weight: 38 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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Database links
- Entrez Gene: 302 Human
- Entrez Gene: 12306 Mouse
- Entrez Gene: 56611 Rat
- Omim: 151740 Human
- SwissProt: P07355 Human
- SwissProt: P07356 Mouse
- SwissProt: Q07936 Rat
- Unigene: 511605 Human
see all -
Alternative names
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
see all
Images
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Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Lanes 1-3 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/20000 dilution
Lane 4 : Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDaExposure time
Lanes 1-3: 1 minute
Lane 4: 3 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206847-anti-annexin-a2-antibody-epr13052b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206845-anti-annexin-a2-antibody-epr13052b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206844-anti-annexin-a2-antibody-epr13052b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Immunofluorescent analysis of HeLa cells labeling Annexin-2/ANXA2 with ab178677 at 1/100 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-206843-anti-annexin-a2-antibody-epr13052b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)Intracellular flow cytometric analysis of permeabilized K562 cells labeling Annexin-2/ANXA2 with ab178677 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)](https://www.abcam.com/ps/products/178/ab178677/Images/ab178677-6-benefits-of-recombinant-antibodies.png)
Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab178677 has been referenced in 16 publications.
- Hsu CY et al. Characterization and Proteomic Analysis of Endometrial Stromal Cell-Derived Small Extracellular Vesicles. J Clin Endocrinol Metab 106:1516-1529 (2021). PubMed: 33507273
- Zhang Y et al. Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1. Nat Commun 12:1731 (2021). PubMed: 33741962
- Martínez-Greene JA et al. Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography. J Extracell Vesicles 10:e12087 (2021). PubMed: 33936570
- Hernández-Díazcouder A et al. High fructose exposure modifies the amount of adipocyte-secreted microRNAs into extracellular vesicles in supernatants and plasma. PeerJ 9:e11305 (2021). PubMed: 34055478
- Shen Y et al. Multi-Omics Integrative Analysis Uncovers Molecular Subtypes and mRNAs as Therapeutic Targets for Liver Cancer. Front Med (Lausanne) 8:654635 (2021). PubMed: 34109194