Recombinant Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free (ab232476)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free
See all Annexin-2/ANXA2 primary antibodies -
Description
Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver tissue.
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General notes
ab232476 is the carrier-free version of ab178677.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13052(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
- Alexa Fluor® 488 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200791)
- Alexa Fluor® 647 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200797)
- HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200798)
- PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab210729)
- Alexa Fluor® 594 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab216386)
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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Database links
- Entrez Gene: 302 Human
- Entrez Gene: 12306 Mouse
- Entrez Gene: 56611 Rat
- Omim: 151740 Human
- SwissProt: P07355 Human
- SwissProt: P07356 Mouse
- SwissProt: Q07936 Rat
- Unigene: 511605 Human
see all -
Alternative names
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178677).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized K562 cells labeling Annexin-2/ANXA2 with ab178677 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178677).
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Annexin-2/ANXA2 with ab178677 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178677).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232476 has not yet been referenced specifically in any publications.