Recombinant HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200798)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]
See all Annexin-2/ANXA2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, SKBR-3 and K562 whole cell lysates. IHC-P: FFPE human prostate (normal) tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13052(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
- Alexa Fluor® 488 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200791)
- Alexa Fluor® 647 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200797)
- PE Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab210729)
- Alexa Fluor® 594 Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab216386)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free (ab232476)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa).
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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Database links
- Entrez Gene: 302 Human
- Entrez Gene: 12306 Mouse
- Entrez Gene: 56611 Rat
- Omim: 151740 Human
- SwissProt: P07355 Human
- SwissProt: P07356 Mouse
- SwissProt: Q07936 Rat
- Unigene: 511605 Human
see all -
Alternative names
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
see all
Images
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IHC image of Annexin-2/ANXA2 staining in a section of formalin-fixed paraffin-embedded human prostate tissue (normal)*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab200798, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200798) at 1/5000 dilution
Lane 1 : SKBR3 (Human adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200798 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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HRP Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab200798) at 1/5000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200798 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab200798 has not yet been referenced specifically in any publications.