Product nameAnti-Annexin-2/ANXA2 antibody [EPR13052(B)] (Phycoerythrin)
See all Annexin-2/ANXA2 primary antibodies
DescriptionRabbit monoclonal [EPR13052(B)] to Annexin-2/ANXA2 (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Annexin-2/ANXA2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P07355
- Flow Cyt: HeLa cells.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (ab178677)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (Alexa Fluor® 488) (ab200791)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (Alexa Fluor® 647) (ab200797)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (HRP) (ab200798)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (Alexa Fluor® 594) (ab216386)
- Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] - BSA and Azide free (ab232476)
Our Abpromise guarantee covers the use of ab210729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF
FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
- Information by UniProt
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
Overlay histogram showing HeLa cells stained with ab210729 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210729, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
ab210729 has not yet been referenced specifically in any publications.