Recombinant Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

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Western blot - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18157-104] to Annexin-2/ANXA2
Suitable for: Flow Cyt, IP, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Annexin-2/ANXA2 antibody [EPR18157-104]
See all Annexin-2/ANXA2 primary antibodies
Description

Rabbit monoclonal [EPR18157-104] to Annexin-2/ANXA2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain, fetal heart, fetal kidney and fetal spleen lysates; HeLa, K562, C6 and NIH/3T3 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat spleen lysate. IHC-P: Mouse liver tissue. Flow Cyt: HeLa cells. IP: NIH/3T3 whole cell lysate.
General notes
This product was previously labelled as Annexin A2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18157-104
Isotype

IgG
Research areas

Alternative Versions
- Anti-Annexin-2/ANXA2 antibody [EPR18157-104] - BSA and Azide free (ab227486)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Annexin-2/ANXA2 protein (ab93005)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
IHC-P	Mouse
IP	Mouse
WB	Mouse Rat Human

Application	Abreviews	Notes
Flow Cyt		1/500.
IP		1/30.
WB		1/5000. Detects a band of approximately 36, 34 kDa (predicted molecular weight: 38 kDa).
IHC-P		1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is not recommended in human or rat due to nonspecific or negative staining.

Notes
Flow Cyt 1/500.
IP 1/30.
WB 1/5000. Detects a band of approximately 36, 34 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is not recommended in human or rat due to nonspecific or negative staining.

Function

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Sequence similarities

Belongs to the annexin family.
Contains 4 annexin repeats.
Domain

A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational
modifications

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated.
Cellular localization

Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Target information above from: UniProt accession P07355 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 302 Human
- Entrez Gene: 12306 Mouse
- Entrez Gene: 56611 Rat
- Omim: 151740 Human
- SwissProt: P07355 Human
- SwissProt: P07356 Mouse
- SwissProt: Q07936 Rat
- Unigene: 511605 Human
- Unigene: 238343 Mouse
- Unigene: 90546 Rat
see all
Alternative names
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
- Annexin-2 antibody
- ANX 2 antibody
- ANX2 antibody
- ANX2L4 antibody
- ANXA2 antibody
- ANXA2_HUMAN antibody
- arylsulfatase B antibody
- CAL1H antibody
- Calpactin I heavy chain antibody
- calpactin I heavy polypeptide (p36) antibody
- Calpactin I heavy polypeptide antibody
- Calpactin-1 heavy chain antibody
- chromobindin 8 antibody
- Chromobindin-8 antibody
- Epididymis secretory protein Li 270 antibody
- HEL S 270 antibody
- LIP2 antibody
- Lipocortin II antibody
- LPC2 antibody
- LPC2D antibody
- p36 antibody
- P36 protein antibody
- PAP-IV antibody
- Placental anticoagulant protein IV antibody
- Protein I antibody
see all

Western blot - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

All lanes : Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Developed using the ECL technique.

Predicted band size: 38 kDa
Observed band size: 36 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

All lanes : Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473) at 1/5000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : C6 (rat glial tumor cell line) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 38 kDa
Observed band size: 34,36 kDa why is the actual band size different from the predicted?

Exposure times: Lane 1: 3 seconds; Lane 2,4: 1 second; Lane 3: 30 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 16373665).
Western blot - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

All lanes : Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473) at 1/5000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 38 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?

Exposure times: Lane 1: 2 seconds; Lanes 2-4: 1 second; Lane 5: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Annexin-2/ANXA2 with ab189473 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the sinusoid of mouse liver (PMID: 23188673). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Annexin-2/ANXA2 with ab189473 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

Annexin-2/ANXA2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab189473 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189473 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).

Lane 2: ab189473 IP in NIH/3T3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189473 in NIH/3T3 whole cell lysate.

Exposure time: 1 second.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Annexin-2/ANXA2 antibody [EPR18157-104] (ab189473)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab189473? Please let us know so that we can cite the reference in this datasheet.

ab189473 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (0)

Customer reviews and Q&As

Post-translational
modifications