Product nameAnnexin-2/ANXA2 overexpression 293T lysate (whole cell)
ab94080 is a 293T cell transfected lysate in which Human Annexin II has been transiently over-expressed using a pCMV-Annexin II plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundDomain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. PTM: Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.
Our Abpromise guarantee covers the use of ab94080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94080 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-Annexin A2 antibody (ab54771) at 1/500 dilution
Lane 1 : Annexin II 293T Transfected Lysate - Positive Control (ab94080)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
ab94080 has not yet been referenced specifically in any publications.