Key features and details
- Rabbit polyclonal to Annexin-4/ANXA4
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Annexin-4/ANXA4 antibody
See all Annexin-4/ANXA4 primary antibodies
DescriptionRabbit polyclonal to Annexin-4/ANXA4
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Annexin-4/ANXA4 aa 1-319.
- WB: HepG2 whole cell lysate. IP: HepG2 whole cell lysate. ICC/IF: HeLa cells. IHC-P: D54MG xenograft.
This product was previously labelled as Annexin IV/ANXA4
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Information by UniProt
- 35 beta calcimedin antibody
- 35-beta calcimedin antibody
- AIV antibody
Anti-Annexin-4/ANXA4 antibody (ab153883) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded D54MG xenograft labeling Annexin IV/ANXA4 with ab153883 at 1/500 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Annexin IV/ANXA4 with ab153883 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Annexin-4/ANXA4 was immunoprecipitated from HepG2 whole cell lysate using 5 μg ab153883.
The WB was probed with ab153883.
Lane 1: Control IgG IP.
Lane 2: ab153883 IP.
ab153883 has not yet been referenced specifically in any publications.