Product nameAnti-Annexin-4/ANXA4 antibody
See all Annexin-4/ANXA4 primary antibodies
DescriptionRabbit polyclonal to Annexin-4/ANXA4
Tested applicationsSuitable for: IHC-Fr, ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
- WB: Placental lysate. IHC-P: Human intestine.
This product was previously labelled as Annexin IV/ANXA4
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Information by UniProt
- 35 beta calcimedin antibody
- 35-beta calcimedin antibody
- AIV antibody
Anti-Annexin-4/ANXA4 antibody (ab33009) at 1.25 µg/ml + Placental lysate
HRP conjugated anti Rabbit IgG 1/50000 - 1/100000
Predicted band size: 36 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelled at Annexin IV/ANXA4 with ab33009 at 4-8µg/ml. Positively labelled cells (indicated by arrows) are epithelial cells of intestinal villi. Magnification: 400X.
This product has been referenced in:
- Choi CH et al. Prognostic significance of annexin A2 and annexin A4 expression in patients with cervical cancer. BMC Cancer 16:448 (2016). Read more (PubMed: 27402115) »
- Chen W et al. Overexpression of annexin A4 indicates poor prognosis and promotes tumor metastasis of hepatocellular carcinoma. Tumour Biol 37:9343-55 (2016). Read more (PubMed: 26779633) »