Overview

  • Product name

    Anti-Annexin-6/ANXA6 antibody
    See all Annexin-6/ANXA6 primary antibodies
  • Description

    Rabbit polyclonal to Annexin-6/ANXA6
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total Annexin-6/ANXA6 protein.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human Annexin-6/ANXA6 (N terminal). The exact sequence is proprietary.
    Database link: P08133

  • Positive control

    • K562, THP-1, MCF7, Mouse spleen, mouse heart, mouse liver, mouse skeletal muscle and mouse lung extracts; Human thyroid cancer, mouse liver and Human lung tissues; A549 cells.
  • General notes

     This product was previously labelled as Annexin VI

     

Properties

Applications

Our Abpromise guarantee covers the use of ab196942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 76 kDa.
IHC-P 1/50 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 8 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylated in response to growth factor stimulation.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 67 kDa calelectrin antibody
    • Annexin A6 antibody
    • Annexin VI antibody
    • Annexin VI p68 antibody
    • Annexin-6 antibody
    • AnnexinA6 antibody
    • AnnexinVI antibody
    • ANX 6 antibody
    • ANX A6 antibody
    • ANX6 antibody
    • ANXA 6 antibody
    • ANXA6 antibody
    • ANXA6_HUMAN antibody
    • Calcium binding protein p68 antibody
    • Calelectrin antibody
    • Calphobindin II antibody
    • Calphobindin-II antibody
    • CalphobindinII antibody
    • CBP 68 antibody
    • CBP68 antibody
    • Chromobindin 20 antibody
    • Chromobindin-20 antibody
    • Chromobindin20 antibody
    • CPB II antibody
    • CPB-II antibody
    • CPBII antibody
    • Lipocortin VI antibody
    • LipocortinVI antibody
    • p68 antibody
    • p70 antibody
    • Protein III antibody
    • ProteinIII antibody
    see all

Images

  • All lanes : Anti-Annexin-6/ANXA6 antibody (ab196942) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : THP-1 cell extract
    Lane 3 : MCF7 cell extract
    Lane 4 : Mouse spleen extract
    Lane 5 : Mouse heart extract
    Lane 6 : Mouse liver extract
    Lane 7 : Mouse skeletal muscle extract
    Lane 8 : Mouse lung extract

    Predicted band size: 76 kDa

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Annexin-6/ANXA6 with ab196942 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Annexin-6/ANXA6 with ab196942 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Annexin-6/ANXA6 with ab196942 at 1/200 dilution.

  • Immunofluorescent analysis of A549 cells labeling Annexin-6/ANXA6 with ab196942 at 1/50 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 26 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cell)
Permeabilization
Yes - NP40
Specification
Vascular smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 26 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MC3T3-E1 murine calvarial osteoblasts (subclone 14)
Permeabilization
Yes - NP40
Specification
MC3T3-E1 murine calvarial osteoblasts (subclone 14
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 20 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MC3T3-E1 murine calvarial osteoblasts (subclone 14)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
MC3T3-E1 murine calvarial osteoblasts (subclone 14
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 20 2018

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