Anti-Annexin-6/ANXA6 antibody (ab196942)
Key features and details
- Rabbit polyclonal to Annexin-6/ANXA6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-6/ANXA6 antibody
See all Annexin-6/ANXA6 primary antibodies -
Description
Rabbit polyclonal to Annexin-6/ANXA6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment within Human Annexin-6/ANXA6 (N terminal). The exact sequence is proprietary.
Database link: P08133 -
Positive control
- K562, THP-1, MCF7, Mouse spleen, mouse heart, mouse liver, mouse skeletal muscle and mouse lung extracts; Human thyroid cancer, mouse liver and Human lung tissues; A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse liver tissue lysate - total protein (ab29301)
- MCF7 whole cell lysate (ab29537)
- Mouse skeletal muscle tissue lysate - total protein (ab29711)
- Mouse heart normal tissue lysate - total protein (ab30291)
- K-562 whole cell lysate (ab7911)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 76 kDa.
|
IHC-P |
1/50 - 1/200.
|
|
ICC/IF | (2) |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 76 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. -
Sequence similarities
Belongs to the annexin family.
Contains 8 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated in response to growth factor stimulation. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 309 Human
- Entrez Gene: 11749 Mouse
- Entrez Gene: 79125 Rat
- Omim: 114070 Human
- SwissProt: P08133 Human
- SwissProt: P14824 Mouse
- SwissProt: P48037 Rat
- Unigene: 412117 Human
see all -
Alternative names
- 67 kDa calelectrin antibody
- Annexin A6 antibody
- Annexin VI antibody
see all
Images
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All lanes : Anti-Annexin-6/ANXA6 antibody (ab196942) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : THP-1 cell extract
Lane 3 : MCF7 cell extract
Lane 4 : Mouse spleen extract
Lane 5 : Mouse heart extract
Lane 6 : Mouse liver extract
Lane 7 : Mouse skeletal muscle extract
Lane 8 : Mouse lung extract
Predicted band size: 76 kDa -
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Annexin-6/ANXA6 with ab196942 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Annexin-6/ANXA6 with ab196942 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Annexin-6/ANXA6 with ab196942 at 1/200 dilution.
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Immunofluorescent analysis of A549 cells labeling Annexin-6/ANXA6 with ab196942 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196942 has been referenced in 1 publication.
- Heinick A et al. Annexin A4 is a novel direct regulator of adenylyl cyclase type 5. FASEB J 29:3773-87 (2015). PubMed: 26023182