Anti-Annexin-6/ANXA6 antibody (ab31026)
- Datasheet
- References (13)
- Protocols
Overview
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Product name
Anti-Annexin-6/ANXA6 antibody
See all Annexin-6/ANXA6 primary antibodies -
Description
Rabbit polyclonal to Annexin-6/ANXA6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Annexin-6/ANXA6 aa 1-100. The exact sequence is proprietary.
Database link: P08133 -
Positive control
- IHC-P: Human L8 cells. Liver.
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General notes
This product was previously labelled as Annexin VI
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). | |
| IHC-P | 1/50. 1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Target
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Function
May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. -
Sequence similarities
Belongs to the annexin family.
Contains 8 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated in response to growth factor stimulation. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 309 Human
- Entrez Gene: 11749 Mouse
- Entrez Gene: 79125 Rat
- Omim: 114070 Human
- SwissProt: P08133 Human
- SwissProt: P14824 Mouse
- SwissProt: P48037 Rat
- Unigene: 412117 Human
see all -
Alternative names
- 67 kDa calelectrin antibody
- Annexin A6 antibody
- Annexin VI antibody
see all
Images
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)This image is courtesy of an anonymous AbreviewAnti-Annexin-6/ANXA6 antibody (ab31026) at 1/500 dilution + Mouse heart tissue lysate at 50 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-6/ANXA6 antibody (ab31026)Ab31026, at a dilution of 1/50, staining formalin fixed paraffin embedded Annexin-6/ANXA6 in human liver tissue by Immunohistochemistry.
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)All lanes : Anti-Annexin-6/ANXA6 antibody (ab31026) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)Anti-Annexin-6/ANXA6 antibody (ab31026) at 1/25 dilution + lysate prepared from 293 cells
Predicted band size: 76 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
References
This product has been referenced in:
- Shimura M et al. A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. Oncotarget 9:24291-24303 (2018). Read more (PubMed: 29849941) »
- Spinelli C et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). Read more (PubMed: 30034643) »
