Western blot abreview for Anti-Annexin-6/ANXA6 antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - nuclear (rat ventricular cardiomyocyte)
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Loading amount
20 µg
Treatment
1. No treatment 2. Endothelin-1 100nM 48hrs
Specification
rat ventricular cardiomyocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 30 second(s)
Bands
Specific: 70 kDa
Positive control
Lane1 : Control no treatment Lane2: Endothelin-1 for 48 hrs

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Submitted Feb 23 2015

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