Recombinant
RabMAb

Recombinant Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)

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Overview

  • Product name

    Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal
    See all Annexin-6/ANXA6 primary antibodies

  • Description

    Rabbit monoclonal [EPR17308] to Annexin-6/ANXA6 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Annexin-6/ANXA6 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P08133

  • Positive control

    • WB: Raji, HeLa and Jurkat whole cell lysate. Mouse brain, kidney and spleen tissue lysate. Rat brain, heart, kidney and spleen tissue lysate. ICC/IF: HeLa and K562 cells.

  • General notes

     

     

     This product was previously labelled as Annexin VI

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 76 kDa).

Target

  • Function

    May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

  • Sequence similarities

    Belongs to the annexin family.
    Contains 8 annexin repeats.

  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.

  • Post-translational
    modifications

    Phosphorylated in response to growth factor stimulation.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 67 kDa calelectrin antibody
    • Annexin A6 antibody
    • Annexin VI antibody
    • Annexin VI p68 antibody
    • Annexin-6 antibody
    • AnnexinA6 antibody
    • AnnexinVI antibody
    • ANX 6 antibody
    • ANX A6 antibody
    • ANX6 antibody
    • ANXA 6 antibody
    • ANXA6 antibody
    • ANXA6_HUMAN antibody
    • Calcium binding protein p68 antibody
    • Calelectrin antibody
    • Calphobindin II antibody
    • Calphobindin-II antibody
    • CalphobindinII antibody
    • CBP 68 antibody
    • CBP68 antibody
    • Chromobindin 20 antibody
    • Chromobindin-20 antibody
    • Chromobindin20 antibody
    • CPB II antibody
    • CPB-II antibody
    • CPBII antibody
    • Lipocortin VI antibody
    • LipocortinVI antibody
    • p68 antibody
    • p70 antibody
    • Protein III antibody
    • ProteinIII antibody
    see all

Images

  • Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    All lanes : Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422) at 1/10000 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 76 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer was 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:12140262).

  • Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    Western blot - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    All lanes : Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 9 : NIH/3T3 (Mouse embryo fibroblast) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 76 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer was 5% NFDM/TBST.
    The observed MW is consistent with what has been described in the literature (PMID:12140262).

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling Annexin-6/ANXA6 with ab199422 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on K562 cell line is observed (Subcellular Location: Cytoplasm [UniProt]). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).

    The negative controls are as follows:-
    -ve control 1 - ab199422 at 1/1000 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Annexin-6/ANXA6 with ab199422 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on HeLa cell line is observed (Subcellular Location: Cytoplasm [UniProt]). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).
    The negative controls are as follows:-
    -ve control 1 - ab199422 at 1/1000 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

  • Immunoprecipitation - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)
    Immunoprecipitation - Anti-Annexin-6/ANXA6 antibody [EPR17308] - N-terminal (ab199422)

    Annexin-6/ANXA6 was immunoprecipitated from 1mg of Raji (Human Burkitt's lymphoma cell line) whole cell extract with ab199422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199422 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Raji whole cell extract 10 µg (Input).

    Lane 2: ab199422 IP in Raji whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199422 in Raji whole cell extract.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab199422 has not yet been referenced specifically in any publications.

Publishing research using ab199422? Please let us know so that we can cite the reference in this datasheet.

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