Recombinant Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
![Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-241335-anti-annexin-vii-antibody-epr16173-n-terminal-western-blot.jpg "Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - N-terminal
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal
See all Annexin-7/ANXA7 primary antibodies -
Description
Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, Human fetal kidney, Human spleen, mouse kidney, mouse spleen, rat kidney and Jurkat lysate. Human cervix carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16173 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/50.
|
|
| IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/5000. Predicted molecular weight: 53 kDa.
|
| Notes |
|---|
|
IP
1/50. |
|
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/5000. Predicted molecular weight: 53 kDa. |
Target
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. -
Tissue specificity
Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. - Information by UniProt
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Database links
- Entrez Gene: 310 Human
- Entrez Gene: 11750 Mouse
- Entrez Gene: 155423 Rat
- Omim: 186360 Human
- SwissProt: P20073 Human
- SwissProt: Q07076 Mouse
- Unigene: 631827 Human
- Unigene: 705617 Human
see all -
Alternative names
- Annexin A7 antibody
- Annexin VII antibody
- Annexin-7 antibody
see all
Images
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Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + U87-MG cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-241334-anti-annexin-vii-antibody-epr16173-n-terminal-western-blot.jpg)
Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + Jurkat cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-241333-anti-annexin-vii-antibody-epr16173-n-terminal-western-blot.jpg)
Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa -
![Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-241332-anti-annexin-vii-antibody-epr16173-n-terminal-western-blot.jpg)
Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-241331-anti-annexin-vii-antibody-epr16173-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-241330-anti-annexin-vii-antibody-epr16173-n-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 µg.
Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)](https://www.abcam.com/ps/products/198/ab198990/Images/ab198990-7-benefits-of-recombinant-antibodies.png)
Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab198990 has not yet been referenced specifically in any publications.
