RecombinantRabMAb

Recombinant Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

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Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
  • Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
  • Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - N-terminal
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal
    See all Annexin-7/ANXA7 primary antibodies

  • Description

    Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Annexin-7/ANXA7 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: P20073

  • Positive control

    • U87-MG, Human fetal kidney, Human spleen, mouse kidney, mouse spleen, rat kidney and Jurkat lysate. Human cervix carcinoma tissue.

  • General notes

    This product was previously labelled as Annexin VII

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab198990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Predicted molecular weight: 53 kDa.

Target

Images

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + U87-MG cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 µg.
    Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

References (0)

Publishing research using ab198990? Please let us know so that we can cite the reference in this datasheet.

ab198990 has not yet been referenced specifically in any publications.

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