Recombinant
RabMAb

Recombinant Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

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Overview

  • Product name

    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal
    See all Annexin-7/ANXA7 primary antibodies

  • Description

    Rabbit monoclonal [EPR16173] to Annexin-7/ANXA7 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Annexin-7/ANXA7 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: P20073

  • Positive control

    • U87-MG, Human fetal kidney, Human spleen, mouse kidney, mouse spleen, rat kidney and Jurkat lysate. Human cervix carcinoma tissue.

  • General notes

    This product was previously labelled as Annexin VII

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Predicted molecular weight: 53 kDa.

Target

Images

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + U87-MG cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

  • Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    All lanes : Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)
    Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

    ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 µg.
    Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab198990 has not yet been referenced specifically in any publications.

Publishing research using ab198990? Please let us know so that we can cite the reference in this datasheet.

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