Overview

  • Product name

    Anti-Annexin-7/ANXA7 antibody [EPR17090]
    See all Annexin-7/ANXA7 primary antibodies
  • Description

    Rabbit monoclonal [EPR17090] to Annexin-7/ANXA7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Annexin-7/ANXA7 aa 1-250. The exact sequence is proprietary.
    Database link: P20073

  • Positive control

    • Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Rat brain lysate, C6 whole cell lysate, Raw264.7 whole cell lysate, PC-12 whole cell lysate, NIH/3T3 whole cell lysate, Human cervix carcinoma tissue, Mouse liver tissue, Neuro-2a cells, SH-SY5Y cells.
  • General notes

     This product was previously labelled as Annexin VII

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • All lanes : Anti-Annexin-7/ANXA7 antibody [EPR17090] (ab197586) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 5 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer was 5% NFDM /TBST

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling ANXA7 with ab197586 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on Neuro-2a cell line is observed. Subcellular location is citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.] The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).

    The negative controls are as follows:-
    -ve control 1 - ab179586 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling ANXA7 with ab197586 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on SH-SY5Y cell line is observed. Subcellular location is citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.] The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).

    The negative controls are as follows:-
    -ve control 1 - ab179586 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

     

  • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling ANXA7 with ab197586 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cervix carcinoma tissue is observed. Subcellular location citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.]. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ANXA7 with ab197586 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed. Subcellular location citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.]. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma from bone marrow) cells labeling ANXA7 with ab197586 at 1/60  (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Lin QS  et al. Annexin A7 induction of neuronal apoptosis via effect on glutamate release in a rat model of subarachnoid hemorrhage. J Neurosurg N/A:1-11 (2019). Read more (PubMed: 30717037) »
  • Heinick A  et al. Annexin A4 is a novel direct regulator of adenylyl cyclase type 5. FASEB J 29:3773-87 (2015). Read more (PubMed: 26023182) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197586.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up