Anti-Annexin A1/ANXA1 antibody (ab88865)
Key features and details
- Mouse polyclonal to Annexin A1/ANXA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Annexin A1/ANXA1 antibody
See all Annexin A1/ANXA1 primary antibodies -
Description
Mouse polyclonal to Annexin A1/ANXA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Annexin A1/ANXA1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human spleen tissue lysate, Annexin A1 transfected 293T cell lysate, Human endometrium tissue and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P | ||
ICC/IF |
Notes |
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IHC-P: Use at a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method in 1X citrate buffer (pH 6.0) before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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Database links
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
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Alternative names
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
Images
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Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 39 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml
Lane 1 : Annexin A1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
ab88865, at 3 µg/ml staining Annexin A1 in formalin-fixed, paraffin-embedded human endometrium by Immunohistochemistry.
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ab88865, at 10 µg/ml staining Annexin A1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab88865 has been referenced in 3 publications.
- Zhang W et al. Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner. Cell Death Dis 12:102 (2021). PubMed: 33473125
- Li L et al. ANXA4 Activates JAK-STAT3 Signaling by Interacting with ANXA1 in Basal-Like Breast Cancer. DNA Cell Biol 39:1649-1656 (2020). PubMed: 32552056
- Marg A et al. Sarcolemmal repair is a slow process and includes EHD2. Traffic 13:1286-94 (2012). PubMed: 22679923