Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
Key features and details
- Mouse monoclonal [2F1] to Annexin A1/ANXA1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Annexin A1/ANXA1 antibody [2F1]
See all Annexin A1/ANXA1 primary antibodies -
Description
Mouse monoclonal [2F1] to Annexin A1/ANXA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 100-250.
Database link: P04083 -
Positive control
- Human Annexin A1 recombinant protein; HeLa, A549, K562, NIH 3T3, C6 and COS7 cell lysates; Human cervical and ovarian cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 39 kDa. |
IHC-P
1/200 - 1/1000. |
Target
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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Database links
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 25380 Rat
- Omim: 151690 Human
- SwissProt: P04083 Human
- SwissProt: P10107 Mouse
- SwissProt: P07150 Rat
- Unigene: 494173 Human
see all -
Alternative names
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
Images
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin A1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab135256 observed at 38 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab135256 was shown to specifically react with Annexin A1 when Annexin A1 knockout samples were used. Wild-type and Annexin A1 knockout samples were subjected to SDS-PAGE. Ab135256 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
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Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution + Human Annexin A1 recombinant protein.
Predicted band size: 39 kDa
Expected MW of recombinant fragment is 33.7 kDa. -
All lanes : Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : COS7 cell lysate
Predicted band size: 39 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab135256 has not yet been referenced specifically in any publications.