Key features and details
- Mouse monoclonal [2F1] to Annexin A1/ANXA1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Product nameAnti-Annexin A1/ANXA1 antibody [2F1]
See all Annexin A1/ANXA1 primary antibodies
DescriptionMouse monoclonal [2F1] to Annexin A1/ANXA1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, African green monkey
Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 144-248.
- Human Annexin A1 recombinant protein; HeLa, A549, K562, NIH 3T3, C6 and COS7 cell lysates; Human cervical and ovarian cancer tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer.
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab135256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/200 - 1/1000.|
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
- Information by UniProt
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin A1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab135256 observed at 38 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab135256 was shown to specifically react with Annexin A1 when Annexin A1 knockout samples were used. Wild-type and Annexin A1 knockout samples were subjected to SDS-PAGE. Ab135256 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution + Human Annexin A1 recombinant protein.
Predicted band size: 39 kDa
Expected MW of recombinant fragment is 33.7 kDa.
All lanes : Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : COS7 cell lysate
Predicted band size: 39 kDa
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
ab135256 has not yet been referenced specifically in any publications.