Anti-Annexin A1/ANXA1 antibody (ab137745)
Key features and details
- Rabbit polyclonal to Annexin A1/ANXA1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Annexin A1/ANXA1 antibody
See all Annexin A1/ANXA1 primary antibodies -
Description
Rabbit polyclonal to Annexin A1/ANXA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 24-346 (internal sequence).
Database link: P04083 -
Positive control
- A431, H1299, HeLa, MOLT4, NIH-3T3 and JC whole cell lysates; HeLa cells; N87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. |
Target
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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Database links
- Entrez Gene: 327662 Cow
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 396942 Pig
- Entrez Gene: 100328962 Rabbit
- Entrez Gene: 25380 Rat
- Omim: 151690 Human
- SwissProt: P46193 Cow
see all -
Alternative names
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
Images
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All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/30000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : Annexin A1/ANXA1 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDa10% SDS-PAGE
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All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : H1299 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution
Lane 1 : NIH-3T3 cell lysate
Lane 2 : JC cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Annexin A1 with ab137745 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded N87 Xenograft, labelling Annexin A1 using ab137745 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137745 has been referenced in 2 publications.
- Klein L et al. Prenatal administration of multipotent adult progenitor cells modulates the systemic and cerebral immune response in an ovine model of chorioamnionitis. Brain Behav Immun Health 23:100458 (2022). PubMed: 35647567
- Fang Y et al. Knockdown of ANXA1 suppresses the biological behavior of human NSCLC cells in vitro. Mol Med Rep 13:3858-66 (2016). WB ; Human . PubMed: 27035116