Anti-Annexin A1/ANXA1 antibody (ab196830)
Key features and details
- Rabbit polyclonal to Annexin A1/ANXA1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Annexin A1/ANXA1 antibody
See all Annexin A1/ANXA1 primary antibodies -
Description
Rabbit polyclonal to Annexin A1/ANXA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human Annexin A1/ANXA1 aa 1 to the C-terminus.
Database link: P04083 -
Positive control
- HeLa cells and cell lysates. NIH/3T3 cell lysates. Human endometrial cancer tissue, Human thyroid cancer tissue and Rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 39 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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Database links
- Entrez Gene: 472953 Chimpanzee
- Entrez Gene: 327662 Cow
- Entrez Gene: 100033940 Horse
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 100171684 Orangutan
- Entrez Gene: 100328962 Rabbit
- Entrez Gene: 25380 Rat
see all -
Alternative names
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
Images
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All lanes : Anti-Annexin A1/ANXA1 antibody (ab196830) at 1/500 dilution
Lane 1 : HeLa cell lysates
Lane 2 : NIH/3T3 cell lysates
Predicted band size: 39 kDa -
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Annexin A1 with ab196830 at 1/200 dilution (400x lens).
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Annexin A1 with ab196830 at 1/200 dilution (200x lens).
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Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Annexin A1 with ab196830 at 1/200 dilution (200x lens).
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Immunofluorescent analysis of HeLa cells labeling Annexin A1 with ab196830 at 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab196830 has been referenced in 2 publications.
- Zhu JF et al. Annexin A1-suppressed autophagy promotes nasopharyngeal carcinoma cell invasion and metastasis by PI3K/AKT signaling activation. Cell Death Dis 9:1154 (2018). PubMed: 30459351
- Gardner PJ et al. Augmenting Endogenous Levels of Retinal Annexin A1 Suppresses Uveitis in Mice. Transl Vis Sci Technol 6:10 (2017). PubMed: 29057162