Overview

  • Product name

    Anti-Annexin A1/ANXA1 antibody
    See all Annexin A1/ANXA1 primary antibodies
  • Description

    Goat polyclonal to Annexin A1/ANXA1
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Annexin A1/ANXA1 aa 324-337 (C terminal).
    Sequence:

    CQAILDETKGDYEK

  • Positive control

    • Recombinant Human Annexin A1 protein (ab86446) can be used as a positive control in WB. A431 lysate Rat spleen

Properties

Applications

Our Abpromise guarantee covers the use of ab81528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1).

Target

  • Function

    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localization

    Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links

  • Alternative names

    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Images

  • All lanes : Anti-Annexin A1/ANXA1 antibody (ab81528) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : NIH3T3 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 39 kDa

  • Anti-Annexin A1/ANXA1 antibody (ab81528) at 1 µg/ml + Pig spleen lysate at 35 µg

    Predicted band size: 39 kDa

  • Anti-Annexin A1/ANXA1 antibody (ab81528) at 1 µg/ml (for 1 hour) + Rat spleen lysate in RIPA buffer at 35 µg

    Predicted band size: 39 kDa

  • Anti-Annexin A1/ANXA1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg

    Predicted band size: 39 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

References

ab81528 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount
30 µg
Specification
protein lysate from lymph node
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Angela Moreno

Verified customer

Submitted Mar 02 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up