Key features and details
- Mouse polyclonal to Annexin A1/ANXA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Annexin A1/ANXA1 antibody
See all Annexin A1/ANXA1 primary antibodies
DescriptionMouse polyclonal to Annexin A1/ANXA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human Annexin A1/ANXA1.
- Human spleen tissue lysate, Annexin A1 transfected 293T cell lysate, Human endometrium tissue and HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method in 1X citrate buffer (pH 6.0) before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
- Information by UniProt
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 39 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
All lanes : Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml
Lane 1 : Annexin A1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 39 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab88865, at 3 µg/ml staining Annexin A1 in formalin-fixed, paraffin-embedded human endometrium by Immunohistochemistry.
ab88865, at 10 µg/ml staining Annexin A1 in HeLa cells by Immunofluorescence.
ab88865 has been referenced in 1 publication.
- Marg A et al. Sarcolemmal repair is a slow process and includes EHD2. Traffic 13:1286-94 (2012). PubMed: 22679923