Overview

  • Product name

    Anti-Annexin A1/ANXA1 antibody
    See all Annexin A1/ANXA1 primary antibodies
  • Description

    Mouse polyclonal to Annexin A1/ANXA1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Annexin A1/ANXA1.

  • Positive control

    • Human spleen tissue lysate, Annexin A1 transfected 293T cell lysate, Human endometrium tissue and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method in 1X citrate buffer (pH 6.0) before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
    • Sequence similarities

      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain

      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      modifications

      Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
    • Cellular localization

      Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
    • Information by UniProt
    • Database links

    • Alternative names

      • Annexin 1 antibody
      • Annexin A1 antibody
      • Annexin I (lipocortin I) antibody
      • Annexin I antibody
      • Annexin-1 antibody
      • AnnexinA1 antibody
      • AnnexinI antibody
      • ANX 1 antibody
      • ANX A1 antibody
      • ANX1 antibody
      • ANXA 1 antibody
      • ANXA1 antibody
      • ANXA1 protein antibody
      • ANXA1_HUMAN antibody
      • Calpactin 2 antibody
      • Calpactin II antibody
      • Calpactin-2 antibody
      • CalpactinII antibody
      • Chromobindin 9 antibody
      • Chromobindin-9 antibody
      • Chromobindin9 antibody
      • HGNC:533 antibody
      • Lipocortin 1 antibody
      • Lipocortin I antibody
      • Lipocortin1 antibody
      • LipocortinI antibody
      • LPC 1 antibody
      • LPC1 antibody
      • p35 antibody
      • Phospholipase A2 inhibitory protein antibody
      see all

    Images

    • Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg

      Predicted band size: 39 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

    • All lanes : Anti-Annexin A1/ANXA1 antibody (ab88865) at 1 µg/ml

      Lane 1 : Annexin A1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 39 kDa
      Observed band size: 36 kDa why is the actual band size different from the predicted?

    • ab88865, at 3 µg/ml staining Annexin A1 in formalin-fixed, paraffin-embedded human endometrium by Immunohistochemistry.
    • ab88865, at 10 µg/ml staining Annexin A1 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    See 1 Publication for this product

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