Overview

  • Product name

    Anti-Annexin A1/ANXA1 antibody [EPR2767(2)]
    See all Annexin A1/ANXA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Annexin A1/ANXA1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P04083

  • Positive control

    • C6, Raw264.7, NIH3T3, C2C12, K562, A431, and HeLa whole cell lysate (ab150035)
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
    • Sequence similarities

      Belongs to the annexin family.
      Contains 4 annexin repeats.
    • Domain

      A pair of annexin repeats may form one binding site for calcium and phospholipid.
    • Post-translational
      modifications

      Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
    • Cellular localization

      Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
    • Information by UniProt
    • Database links

    • Alternative names

      • Annexin 1 antibody
      • Annexin A1 antibody
      • Annexin I (lipocortin I) antibody
      • Annexin I antibody
      • Annexin-1 antibody
      • AnnexinA1 antibody
      • AnnexinI antibody
      • ANX 1 antibody
      • ANX A1 antibody
      • ANX1 antibody
      • ANXA 1 antibody
      • ANXA1 antibody
      • ANXA1 protein antibody
      • ANXA1_HUMAN antibody
      • Calpactin 2 antibody
      • Calpactin II antibody
      • Calpactin-2 antibody
      • CalpactinII antibody
      • Chromobindin 9 antibody
      • Chromobindin-9 antibody
      • Chromobindin9 antibody
      • HGNC:533 antibody
      • Lipocortin 1 antibody
      • Lipocortin I antibody
      • Lipocortin1 antibody
      • LipocortinI antibody
      • LPC 1 antibody
      • LPC1 antibody
      • p35 antibody
      • Phospholipase A2 inhibitory protein antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Annexin I knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

       

      Lanes 1 - 3: Merged signal (red and green). Green - ab138512 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

       

      ab138512 was shown to specifically react with Annexin I when Annexin I knockout samples were used. Wild-type and Annexin I knockout samples were subjected to SDS-PAGE. Ab138512 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : NIH3T3 cell lysate
      Lane 4 : C2C12 cell lysate
      Lane 5 : K562 cell lysate
      Lane 6 : A431 cell lysate
      Lane 7 : HeLa cell lysate

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    References

    This product has been referenced in:

    • Yuan Y  et al. Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model. Sci Rep 5:9314 (2015). WB, IHC-P ; Rat . Read more (PubMed: 25791774) »
    See 1 Publication for this product

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