Product nameAnti-Annexin A1/ANXA1 antibody [EPR2767(2)]
See all Annexin A1/ANXA1 primary antibodies
DescriptionRabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Annexin A1/ANXA1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P04083
- C6, Raw264.7, NIH3T3, C2C12, K562, A431, and HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab138512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).|
FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
- Information by UniProt
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin I knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab138512 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab138512 was shown to specifically react with Annexin I when Annexin I knockout samples were used. Wild-type and Annexin I knockout samples were subjected to SDS-PAGE. Ab138512 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : HeLa cell lysate
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa