Overview

  • Product name
  • Description
    Goat polyclonal to Annexin A2
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognise both reported isoforms. NP_004030.1, NP_001002857.1and NP_001129487.1 are varients of isoform 2.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    STVHEILCKLSLEGD

    , corresponding to N terminal amino acids 2-16 of Human Annexin II.

  • Positive control
    • HeLa and A549 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab22880).

Target

  • Function
    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities
    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Images

  • Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    Primary incubated for 1 hour.

References

This product has been referenced in:
  • Ribeiro-Oliveira A  et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . Read more (PubMed: 18701577) »
  • Glebov OO  et al. Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54 (2006). Read more (PubMed: 16341206) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (pig brain lysate and pig endothelial cell line lys)
Loading amount
10 µg
Specification
pig brain lysate and pig endothelial cell line lys
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Abcam user community

Verified customer

Submitted Aug 04 2006

Answer

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC-P. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image).

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Answer

Thank you for your email. It does sound like the problem was with this primary, especially as you successfully used a different annexin II primary under the same conditions. I have instructed our accounting department to issue you a credit for your order for ab2242. If you have any questions regarding the credit, please contact accounts@abcam.com If I can be of additional assistance please let me know.

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Answer

Thank you for your enquiry and patience, and I'm sorry to hear that you are experiencing difficulty with ab2242. We have not had any complaints regarding this antibody, and so there is a possibility that the vial you received went off during the shipping process. Can you please just confirm that the secondary antibody that you used along with ab2242 is working properly? I can offer you a replacement vial or a credit, please let me know which you would prefer.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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